Cloning and characterization of a functional promoter of the human SOCS-3 gene

Biao He; Liang You; Kazutsugu Uematsu; Maria Matsangou; Zhidong Xu; Miao He; Frank McCormick; David M. Jablons

doi:10.1016/S0006-291X(02)03071-1

Cloning and characterization of a functional promoter of the human SOCS-3 gene

Biao He, Liang You, Kazutsugu Uematsu, Maria Matsangou, Zhidong Xu, Miao He, Frank McCormick, David M. Jablons^*

^*Corresponding author for this work

Medicine, Hematology Oncology Division

Research output: Contribution to journal › Article › peer-review

61 Scopus citations

Abstract

SOCS-3 is a member of a newly discovered protein family that inhibits LIF-activated Janus kinase (JAK)-signal transducers and activators of transcription (STAT) signaling in a negative auto-regulatory manner. In this study, we have cloned and characterized the promoter region of the human SOCS-3 gene. This region is ∼1.1kbp in length and consists of two putative STAT-binding elements, a G-rich element, and a putative TATA box. These elements are highly conserved in both murine and rat SOCS-3 promoters. Functional analysis of this region shows that the whole fragment (∼1.1kbp) has high basal promoter activity and is responsive to growth factors. We also found that the wild type SOCS-3 promoter construct has significantly greater activity in non-small-cell lung cancer cell lines than in normal cells in accordance with STAT3 disregulation in these cells. Cloning of the human SOCS-3 promoter should help uncover mechanisms of regulation of the JAK-STAT pathway in human cancer.

Original language	English (US)
Pages (from-to)	386-391
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	301
Issue number	2
DOIs	https://doi.org/10.1016/S0006-291X(02)03071-1
State	Published - Feb 7 2003

Funding

This work was supported by the Larry Hall memorial trust and the Kazan, McClain, Edises, Abrams, Fernandez, Lyons & Farrise Foundation.

Keywords

293T cells
Human
Lung cancer
Promoter
SOCS-3
STAT3

ASJC Scopus subject areas

Molecular Biology
Biophysics
Biochemistry
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/S0006-291X(02)03071-1

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title = "Cloning and characterization of a functional promoter of the human SOCS-3 gene",

abstract = "SOCS-3 is a member of a newly discovered protein family that inhibits LIF-activated Janus kinase (JAK)-signal transducers and activators of transcription (STAT) signaling in a negative auto-regulatory manner. In this study, we have cloned and characterized the promoter region of the human SOCS-3 gene. This region is ∼1.1kbp in length and consists of two putative STAT-binding elements, a G-rich element, and a putative TATA box. These elements are highly conserved in both murine and rat SOCS-3 promoters. Functional analysis of this region shows that the whole fragment (∼1.1kbp) has high basal promoter activity and is responsive to growth factors. We also found that the wild type SOCS-3 promoter construct has significantly greater activity in non-small-cell lung cancer cell lines than in normal cells in accordance with STAT3 disregulation in these cells. Cloning of the human SOCS-3 promoter should help uncover mechanisms of regulation of the JAK-STAT pathway in human cancer.",

keywords = "293T cells, Human, Lung cancer, Promoter, SOCS-3, STAT3",

author = "Biao He and Liang You and Kazutsugu Uematsu and Maria Matsangou and Zhidong Xu and Miao He and Frank McCormick and Jablons, {David M.}",

note = "Funding Information: This work was supported by the Larry Hall memorial trust and the Kazan, McClain, Edises, Abrams, Fernandez, Lyons & Farrise Foundation. ",

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T1 - Cloning and characterization of a functional promoter of the human SOCS-3 gene

AU - He, Biao

AU - You, Liang

AU - Uematsu, Kazutsugu

AU - Matsangou, Maria

AU - Xu, Zhidong

AU - He, Miao

AU - McCormick, Frank

AU - Jablons, David M.

N1 - Funding Information: This work was supported by the Larry Hall memorial trust and the Kazan, McClain, Edises, Abrams, Fernandez, Lyons & Farrise Foundation.

PY - 2003/2/7

Y1 - 2003/2/7

N2 - SOCS-3 is a member of a newly discovered protein family that inhibits LIF-activated Janus kinase (JAK)-signal transducers and activators of transcription (STAT) signaling in a negative auto-regulatory manner. In this study, we have cloned and characterized the promoter region of the human SOCS-3 gene. This region is ∼1.1kbp in length and consists of two putative STAT-binding elements, a G-rich element, and a putative TATA box. These elements are highly conserved in both murine and rat SOCS-3 promoters. Functional analysis of this region shows that the whole fragment (∼1.1kbp) has high basal promoter activity and is responsive to growth factors. We also found that the wild type SOCS-3 promoter construct has significantly greater activity in non-small-cell lung cancer cell lines than in normal cells in accordance with STAT3 disregulation in these cells. Cloning of the human SOCS-3 promoter should help uncover mechanisms of regulation of the JAK-STAT pathway in human cancer.

AB - SOCS-3 is a member of a newly discovered protein family that inhibits LIF-activated Janus kinase (JAK)-signal transducers and activators of transcription (STAT) signaling in a negative auto-regulatory manner. In this study, we have cloned and characterized the promoter region of the human SOCS-3 gene. This region is ∼1.1kbp in length and consists of two putative STAT-binding elements, a G-rich element, and a putative TATA box. These elements are highly conserved in both murine and rat SOCS-3 promoters. Functional analysis of this region shows that the whole fragment (∼1.1kbp) has high basal promoter activity and is responsive to growth factors. We also found that the wild type SOCS-3 promoter construct has significantly greater activity in non-small-cell lung cancer cell lines than in normal cells in accordance with STAT3 disregulation in these cells. Cloning of the human SOCS-3 promoter should help uncover mechanisms of regulation of the JAK-STAT pathway in human cancer.

KW - 293T cells

KW - Human

KW - Lung cancer

KW - Promoter

KW - SOCS-3

KW - STAT3

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Cloning and characterization of a functional promoter of the human SOCS-3 gene

Abstract

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

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