Cloning and characterization of a functional promoter of the human SOCS-3 gene

Biao He, Liang You, Kazutsugu Uematsu, Maria Matsangou, Zhidong Xu, Miao He, Frank McCormick, David M. Jablons*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

61 Scopus citations

Abstract

SOCS-3 is a member of a newly discovered protein family that inhibits LIF-activated Janus kinase (JAK)-signal transducers and activators of transcription (STAT) signaling in a negative auto-regulatory manner. In this study, we have cloned and characterized the promoter region of the human SOCS-3 gene. This region is ∼1.1kbp in length and consists of two putative STAT-binding elements, a G-rich element, and a putative TATA box. These elements are highly conserved in both murine and rat SOCS-3 promoters. Functional analysis of this region shows that the whole fragment (∼1.1kbp) has high basal promoter activity and is responsive to growth factors. We also found that the wild type SOCS-3 promoter construct has significantly greater activity in non-small-cell lung cancer cell lines than in normal cells in accordance with STAT3 disregulation in these cells. Cloning of the human SOCS-3 promoter should help uncover mechanisms of regulation of the JAK-STAT pathway in human cancer.

Original languageEnglish (US)
Pages (from-to)386-391
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume301
Issue number2
DOIs
StatePublished - Feb 7 2003

Keywords

  • 293T cells
  • Human
  • Lung cancer
  • Promoter
  • SOCS-3
  • STAT3

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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