Cloning and characterization of cDNA encoding canine α-L-iduronidase. mRNA deficiency in mucopolysaccharidosis I dog

Lori J. Stoltzfus, Beatriz Sosa-Pineda, Samuel M. Moskowitz, Kaushiki P. Menon, Bonnie Dlott, Lucilla Hooper, David B. Teplow, Robert M. Shull, Elizabeth F. Neufeld

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

α-L-Iduronidase is a lysosomal enzyme, the deficiency of which causes mucopolysaccharidosis I (MPS I); a canine MPS I colony has been bred to test therapeutic intervention. The enzyme was purified to apparent homogeneity from canine testis and found to consist of two electrophoretically separable proteins that had common internal peptides but differed at their amino termini. A 57-base oligonucleotide, corresponding to the most probable codons of the longest peptide, was used to screen a canine testis cDNA library. Three cDNAs were isolated, two of which lacked the 5′-end whereas the third was full-length except for a small internal deletion. The composite sequence encodes an open reading frame of 655 amino acids that includes all sequenced peptides. The amino terminus of the larger protein, glutamic acid 26, is at the predicted signal peptide cleavage site, whereas the amino terminus of the smaller protein is leucine 106. There are six potential N-glycosylation sites and a non-canonical polyadenylation signal, CTTAAA. A search of Gen-Bank showed that the amino acid sequence of α-L-iduronidase has similarity to that of a bacterial β-xylosidase. A full-length cDNA corresponding to the composite sequence was constructed (pcIdu) and inserted into the pSVL expression vector (pSVcIdu). Two days after Cos-1 cells were transfected with pSVcIdu, their intracellular and secreted level of α-L-iduronidase activity had increased 8- and 22-fold, respectively, over the endogenous activity. Fibroblasts of MPS I dogs, which have no α-L-iduronidase activity, lacked the normal β-L-iduronidase mRNA of 2.2 kilobases and contained instead a trace amount of a 2.8-kilobase species. Isolation and characterization of an expressible α-L-iduronidase cDNA represents the first step toward mutation analysis and replacement therapy.

Original languageEnglish (US)
Pages (from-to)6570-6575
Number of pages6
JournalJournal of Biological Chemistry
Volume267
Issue number10
StatePublished - 1992

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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