Cloning and characterization of the Aspergillus nidulans cysB gene encoding cysteine synthase

Jacek Topczewski, Marzena Sienko, Andrzej Paszewski*

*Corresponding author for this work

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

The cysB gene of A. nidulans was cloned by complementation of a cysB mutation. This is the first cloned eukaryotic genomic sequence coding for cysteine synthase. The gene contains one 71-bp intron and codes for a protein of 370 amino acids. Its N-terminal region has characteristic features of transit peptides, suggesting mitochondrial localisation of the enzyme. The protein shows homology with bacterial and plant cysteine synthases among which it occupies a remote phylogenetic position and apparently represents a distinct subfamily. Transcription of the cysB gene is not appreciably regulated by the concentration of methionine in the growth medium.

Original languageEnglish (US)
Pages (from-to)348-356
Number of pages9
JournalCurrent Genetics
Volume31
Issue number4
DOIs
StatePublished - Apr 1 1997

Keywords

  • Aspergillus nidulans
  • Cysteine synthase
  • Gene
  • [EC 4.2.99.8]
  • cysB

ASJC Scopus subject areas

  • Genetics

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