Cloning and Expression of Canine Interleukin-10

Ping Lu*, Keith Zucker, Laphalle Fuller, Andreas Tzakis, Violet Esquenazi, Joshua Miller, Laphalle Fuller, Violet Esquenazi, Joshua Miller

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

We describe here the cloning of canine IL-10 cDNA (GenBank accession No. U33843) and the expression of recombinant IL-10 in a dog kidney cell line (DK6247) and Chinese hamster ovary cells (CHO). Canine IL-10 exhibits strong sequence homology to the known sequences of human, mouse, rat, and bovine genes at nucleotide and amino acid levels. The IL-10 gene, when introduced into DK and CHO cell lines, produces recombinant IL-10 that causes an inhibitory effect on allogeneic MHC-driven lymphoproliferative responses.

Original languageEnglish (US)
Pages (from-to)1103-1109
Number of pages7
JournalJournal of Interferon and Cytokine Research
Volume15
Issue number12
DOIs
StatePublished - Dec 1995

ASJC Scopus subject areas

  • Immunology
  • Cell Biology
  • Virology

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