Cloning and identifying renal cell carcinoma differentially expressed genes and their significance

Q. Zhang*, Z. Zhang, L. Chen

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

OBJECTIVE: To Clone study the differentially expressed new genes in renal cell carcinoma (RCC). METHODS: Using a technique known as suppression subtractive hybridization to construct the library which contains the differently expressing cDNAs between RCC and normal kidney cells. Then the RCC specifically expressed genes were cloned. RESULTS: Human RCC subtractive library with high subtractive efficiency was set up successfully. The amplified library contained 350 positive clones. Sequence analysis were performed for 5 clones. All the sequences were unknown previously and the cDNA insert GYLZ-RCC18 had three copies. Northern blot analysis showed that GYLZ-RCC18 cDNA expressed highly in RCC, but no signal could be detected in normal kidney. Using SMART RACE technique, the full length of novel gene of GYLZ-RCC18 was obtained. CONCLUSIONS: The highly efficient cDNA subtractive library may have formed solid foundation for screening and cloning new and specific oncogenes or tumor suppressor genes of RCC. The novel differentially expressed genes may provide an important clue for studying the mechanism of occurrence and development of RCC.

Original languageEnglish (US)
Pages (from-to)356-359
Number of pages4
JournalZhonghua yi xue za zhi
Volume81
Issue number6
StatePublished - Mar 25 2001

ASJC Scopus subject areas

  • Medicine(all)

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