Cloning of a cDNA encoding a human DNA-binding protein similar to ribosomal protein S1

Elizabeth A. Eklund; Seung won Lee; David G. Skalnik

doi:10.1016/0378-1119(94)00898-3

Cloning of a cDNA encoding a human DNA-binding protein similar to ribosomal protein S1

Elizabeth A. Eklund, Seung won Lee, David G. Skalnik^*

^*Corresponding author for this work

Medicine, Hematology Oncology Division

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

We report the cloning of a human complementary DNA that encodes a protein which exhibits 36% identity and 62% similarity to Escherichia coli ribosomal protein S1 (rpS1), including conservation of four copies of an RNA-binding domain. This clone was obtained by ligand-screening a λgt11 expression library with a DNA probe derived from the CYBB gene promoter. Electrophoretic mobility shift and Southwestern blot assays confirm DNA binding activity of the protein, which exhibits preferential binding to single-stranded and double-stranded DNA and a low binding affinity for RNA. Hence, the rpS1 protein domain previously identified as an RNA-binding motif can also serve as a DNA-binding domain.

Original language	English (US)
Pages (from-to)	231-235
Number of pages	5
Journal	Gene
Volume	155
Issue number	2
DOIs	https://doi.org/10.1016/0378-1119(94)00898-3
State	Published - Apr 3 1995

Keywords

Escherichia coli
Glutathione S-transferase fusion protein
Southwestern blot
ligand screen

ASJC Scopus subject areas

Genetics

Access to Document

10.1016/0378-1119(94)00898-3

Cite this

@article{cf95701bfee74d54bacc3b5a3b55f7b1,

title = "Cloning of a cDNA encoding a human DNA-binding protein similar to ribosomal protein S1",

abstract = "We report the cloning of a human complementary DNA that encodes a protein which exhibits 36% identity and 62% similarity to Escherichia coli ribosomal protein S1 (rpS1), including conservation of four copies of an RNA-binding domain. This clone was obtained by ligand-screening a λgt11 expression library with a DNA probe derived from the CYBB gene promoter. Electrophoretic mobility shift and Southwestern blot assays confirm DNA binding activity of the protein, which exhibits preferential binding to single-stranded and double-stranded DNA and a low binding affinity for RNA. Hence, the rpS1 protein domain previously identified as an RNA-binding motif can also serve as a DNA-binding domain.",

keywords = "Escherichia coli, Glutathione S-transferase fusion protein, Southwestern blot, ligand screen",

author = "Eklund, {Elizabeth A.} and Lee, {Seung won} and Skalnik, {David G.}",

note = "Funding Information: We thank Mark Kelley and Ira Wool for helpful discussions regarding multifunctional ribosomal proteins, Yi Xu for providing RNA probes, and Riley Children's Cancer Research for supporting oligonucleotide synthesis. This work was supported by the Riley Memorial Association, and by the following grants awarded to D.G.S.: NIH No. 1 R29 CA58947, American Cancer Society No. JFRA 421, a National Leukemia Association Grant and a grant by the Project Development Program, Research and Sponsored Programs, Indiana University at Indianapolis. E.A.E. and S.-w.L. were supported by the Walther Oncology Center.",

year = "1995",

month = apr,

day = "3",

doi = "10.1016/0378-1119(94)00898-3",

language = "English (US)",

volume = "155",

pages = "231--235",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Cloning of a cDNA encoding a human DNA-binding protein similar to ribosomal protein S1

AU - Eklund, Elizabeth A.

AU - Lee, Seung won

AU - Skalnik, David G.

N1 - Funding Information: We thank Mark Kelley and Ira Wool for helpful discussions regarding multifunctional ribosomal proteins, Yi Xu for providing RNA probes, and Riley Children's Cancer Research for supporting oligonucleotide synthesis. This work was supported by the Riley Memorial Association, and by the following grants awarded to D.G.S.: NIH No. 1 R29 CA58947, American Cancer Society No. JFRA 421, a National Leukemia Association Grant and a grant by the Project Development Program, Research and Sponsored Programs, Indiana University at Indianapolis. E.A.E. and S.-w.L. were supported by the Walther Oncology Center.

PY - 1995/4/3

Y1 - 1995/4/3

N2 - We report the cloning of a human complementary DNA that encodes a protein which exhibits 36% identity and 62% similarity to Escherichia coli ribosomal protein S1 (rpS1), including conservation of four copies of an RNA-binding domain. This clone was obtained by ligand-screening a λgt11 expression library with a DNA probe derived from the CYBB gene promoter. Electrophoretic mobility shift and Southwestern blot assays confirm DNA binding activity of the protein, which exhibits preferential binding to single-stranded and double-stranded DNA and a low binding affinity for RNA. Hence, the rpS1 protein domain previously identified as an RNA-binding motif can also serve as a DNA-binding domain.

AB - We report the cloning of a human complementary DNA that encodes a protein which exhibits 36% identity and 62% similarity to Escherichia coli ribosomal protein S1 (rpS1), including conservation of four copies of an RNA-binding domain. This clone was obtained by ligand-screening a λgt11 expression library with a DNA probe derived from the CYBB gene promoter. Electrophoretic mobility shift and Southwestern blot assays confirm DNA binding activity of the protein, which exhibits preferential binding to single-stranded and double-stranded DNA and a low binding affinity for RNA. Hence, the rpS1 protein domain previously identified as an RNA-binding motif can also serve as a DNA-binding domain.

KW - Escherichia coli

KW - Glutathione S-transferase fusion protein

KW - Southwestern blot

KW - ligand screen

UR - http://www.scopus.com/inward/record.url?scp=0028901673&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028901673&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(94)00898-3

DO - 10.1016/0378-1119(94)00898-3

M3 - Article

C2 - 7721096

AN - SCOPUS:0028901673

SN - 0378-1119

VL - 155

SP - 231

EP - 235

JO - Gene

JF - Gene

IS - 2

ER -

Cloning of a cDNA encoding a human DNA-binding protein similar to ribosomal protein S1

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this