Cloning of a D-type cyclin from murine erythroleukemia cells

Hiroaki Kiyokawa*, Xavier Busquets, C. Thomas Powell, Lang Ngo, Richard A. Rifkind, Paul A. Marks

*Corresponding author for this work

Research output: Contribution to journalArticle

51 Scopus citations

Abstract

We report the complete coding sequence of a cDNA, designated CYL2, derived from a murine erythroleukemia cell library. CYL2 is considered to encode a D- type cyclin because (i) there is cross hybridization with CYL1 (a murine homolog of human cyclin D1) and the encoded protein has 64% amino acid sequence identity with CYL1 and (ii) murine erythroleukemia cell-derived CYL2 contains an amino acid sequence identical to that previously reported for the C-terminal portion of a partially sequenced CYL2. Transcripts of murine erythroleukemia cell CYL2 undergo alternative polyadenylylation like that of human cyclin D1. A major 6.5-kilobase CYL2 transcript changes its expression during the cell cycle with a broad peak through G1 and S phases and a decrease in G2/M phases. The present findings suggest that CYL2 plays a role in the G1 to S phase progression.

Original languageEnglish (US)
Pages (from-to)2444-2447
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume89
Issue number6
DOIs
StatePublished - 1992

Keywords

  • CYL2 cDNA
  • cell cycle

ASJC Scopus subject areas

  • General

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