Cloning of an almost full-length chicken conalbumin double-stranded cDNA

M. Cochet*, F. Perrin, F. Gannon, A. Krust, P. Chambon, G. S. Mcknight, D. C. Lee, K. E. Mayo, R. Palmiter

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

Chicken conalbumin double-stranded cDNA (con-dscDNA) was synthesized from a laying hen oviduct mRNA preparation enriched for conalbumin mRNA (con-mRNA). The dscDNA was inserted by bluntend ligation into the SalI site of plasmid pBR322 which had been repaired with DNA polymerase I to create TaqI sites on each side of the inserted fragment. After bacterial transformation, one hybrid recombinant, pBR322-con1, which contains the largest inserted dscDNA (about 2350 bp) was shown to hybridize specifically to the RNA which is translated into conalbumin. Electron microscopic examination of hybrid molecules between con-mRNA and pBR322-con1 DNA indicate that the inserted con-dscDNA is an almost full-length double-stranded transcript of conalbumin mRNA.

Original languageEnglish (US)
Pages (from-to)2435-2452
Number of pages18
JournalNucleic acids research
Volume6
Issue number7
DOIs
StatePublished - Jun 11 1979

ASJC Scopus subject areas

  • Genetics

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