Cloning of an almost full-length chicken conalbumin double-stranded cDNA

M. Cochet*, F. Perrin, F. Gannon, A. Krust, P. Chambon, G. S. Mcknight, D. C. Lee, K. E. Mayo, R. Palmiter

*Corresponding author for this work

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

Chicken conalbumin double-stranded cDNA (con-dscDNA) was synthesized from a laying hen oviduct mRNA preparation enriched for conalbumin mRNA (con-mRNA). The dscDNA was inserted by bluntend ligation into the SalI site of plasmid pBR322 which had been repaired with DNA polymerase I to create TaqI sites on each side of the inserted fragment. After bacterial transformation, one hybrid recombinant, pBR322-con1, which contains the largest inserted dscDNA (about 2350 bp) was shown to hybridize specifically to the RNA which is translated into conalbumin. Electron microscopic examination of hybrid molecules between con-mRNA and pBR322-con1 DNA indicate that the inserted con-dscDNA is an almost full-length double-stranded transcript of conalbumin mRNA.

Original languageEnglish (US)
Pages (from-to)2435-2452
Number of pages18
JournalNucleic acids research
Volume6
Issue number7
DOIs
StatePublished - Jun 11 1979

Fingerprint

Conalbumin
Organism Cloning
Chickens
Messenger RNA
Bacterial Transformation
DNA Polymerase I
Oviducts
Ligation
Plasmids
Complementary DNA
RNA
Electrons
DNA

ASJC Scopus subject areas

  • Genetics

Cite this

Cochet, M., Perrin, F., Gannon, F., Krust, A., Chambon, P., Mcknight, G. S., ... Palmiter, R. (1979). Cloning of an almost full-length chicken conalbumin double-stranded cDNA. Nucleic acids research, 6(7), 2435-2452. https://doi.org/10.1093/nar/6.7.2435
Cochet, M. ; Perrin, F. ; Gannon, F. ; Krust, A. ; Chambon, P. ; Mcknight, G. S. ; Lee, D. C. ; Mayo, K. E. ; Palmiter, R. / Cloning of an almost full-length chicken conalbumin double-stranded cDNA. In: Nucleic acids research. 1979 ; Vol. 6, No. 7. pp. 2435-2452.
@article{f2dbe3feeb2c46fdb20abef87f30b204,
title = "Cloning of an almost full-length chicken conalbumin double-stranded cDNA",
abstract = "Chicken conalbumin double-stranded cDNA (con-dscDNA) was synthesized from a laying hen oviduct mRNA preparation enriched for conalbumin mRNA (con-mRNA). The dscDNA was inserted by bluntend ligation into the SalI site of plasmid pBR322 which had been repaired with DNA polymerase I to create TaqI sites on each side of the inserted fragment. After bacterial transformation, one hybrid recombinant, pBR322-con1, which contains the largest inserted dscDNA (about 2350 bp) was shown to hybridize specifically to the RNA which is translated into conalbumin. Electron microscopic examination of hybrid molecules between con-mRNA and pBR322-con1 DNA indicate that the inserted con-dscDNA is an almost full-length double-stranded transcript of conalbumin mRNA.",
author = "M. Cochet and F. Perrin and F. Gannon and A. Krust and P. Chambon and Mcknight, {G. S.} and Lee, {D. C.} and Mayo, {K. E.} and R. Palmiter",
year = "1979",
month = "6",
day = "11",
doi = "10.1093/nar/6.7.2435",
language = "English (US)",
volume = "6",
pages = "2435--2452",
journal = "Nucleic Acids Research",
issn = "0305-1048",
publisher = "Oxford University Press",
number = "7",

}

Cochet, M, Perrin, F, Gannon, F, Krust, A, Chambon, P, Mcknight, GS, Lee, DC, Mayo, KE & Palmiter, R 1979, 'Cloning of an almost full-length chicken conalbumin double-stranded cDNA', Nucleic acids research, vol. 6, no. 7, pp. 2435-2452. https://doi.org/10.1093/nar/6.7.2435

Cloning of an almost full-length chicken conalbumin double-stranded cDNA. / Cochet, M.; Perrin, F.; Gannon, F.; Krust, A.; Chambon, P.; Mcknight, G. S.; Lee, D. C.; Mayo, K. E.; Palmiter, R.

In: Nucleic acids research, Vol. 6, No. 7, 11.06.1979, p. 2435-2452.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Cloning of an almost full-length chicken conalbumin double-stranded cDNA

AU - Cochet, M.

AU - Perrin, F.

AU - Gannon, F.

AU - Krust, A.

AU - Chambon, P.

AU - Mcknight, G. S.

AU - Lee, D. C.

AU - Mayo, K. E.

AU - Palmiter, R.

PY - 1979/6/11

Y1 - 1979/6/11

N2 - Chicken conalbumin double-stranded cDNA (con-dscDNA) was synthesized from a laying hen oviduct mRNA preparation enriched for conalbumin mRNA (con-mRNA). The dscDNA was inserted by bluntend ligation into the SalI site of plasmid pBR322 which had been repaired with DNA polymerase I to create TaqI sites on each side of the inserted fragment. After bacterial transformation, one hybrid recombinant, pBR322-con1, which contains the largest inserted dscDNA (about 2350 bp) was shown to hybridize specifically to the RNA which is translated into conalbumin. Electron microscopic examination of hybrid molecules between con-mRNA and pBR322-con1 DNA indicate that the inserted con-dscDNA is an almost full-length double-stranded transcript of conalbumin mRNA.

AB - Chicken conalbumin double-stranded cDNA (con-dscDNA) was synthesized from a laying hen oviduct mRNA preparation enriched for conalbumin mRNA (con-mRNA). The dscDNA was inserted by bluntend ligation into the SalI site of plasmid pBR322 which had been repaired with DNA polymerase I to create TaqI sites on each side of the inserted fragment. After bacterial transformation, one hybrid recombinant, pBR322-con1, which contains the largest inserted dscDNA (about 2350 bp) was shown to hybridize specifically to the RNA which is translated into conalbumin. Electron microscopic examination of hybrid molecules between con-mRNA and pBR322-con1 DNA indicate that the inserted con-dscDNA is an almost full-length double-stranded transcript of conalbumin mRNA.

UR - http://www.scopus.com/inward/record.url?scp=0018788024&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018788024&partnerID=8YFLogxK

U2 - 10.1093/nar/6.7.2435

DO - 10.1093/nar/6.7.2435

M3 - Article

VL - 6

SP - 2435

EP - 2452

JO - Nucleic Acids Research

JF - Nucleic Acids Research

SN - 0305-1048

IS - 7

ER -

Cochet M, Perrin F, Gannon F, Krust A, Chambon P, Mcknight GS et al. Cloning of an almost full-length chicken conalbumin double-stranded cDNA. Nucleic acids research. 1979 Jun 11;6(7):2435-2452. https://doi.org/10.1093/nar/6.7.2435