Cloning of cDNA Encoding Rat Phospholipase C-β4, a New Member of the Phospholipase C

M. J. Kim, Y. Y. Bahk, D. S. Min, S. J. Lee, S. H. Ryu, P. G. Suh

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Phospholipase C-β4(PLC-β4), a new member of phospholipase C isozyme, was purified from bovine cerebellum. The cDNA encoding rat PLC-β4 has been cloned from a cDNA library prepared from rat brain. The predicted open reading frame encodes a protein of 1,176 amino acids with a calculated molecular weight of 134,552. The deduced amino acid sequence exhibits 39, 36, and 36% identity with the sequences of rat PLC-β1, human PLC-β2, and rat PLC-β3, respectively. The amino acid sequence of PLC-β4, especially, shows higher identity (50%) with norpA PLC sequence from Drosophila melanogaster than those of other PLC-β subtypes, suggesting that the PLC-β4 might be a mammalian PLC equivalent of norpA PLC implicated in photosignal transduction in Drosophila.

Original languageEnglish (US)
Pages (from-to)706-712
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume194
Issue number2
DOIs
StatePublished - Jul 30 1993

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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