Cloning of the gene, speB, for streptococcal pyrogenic exotoxin type B in Escherichia coli

G. A. Bohach, A. R. Hauser, P. M. Schlievert

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

The structural gene encoding streptococcal pyrogenic exotoxin type B, designated speB, was cloned in Escherichia coli and localized onto a 4.5-kilobase BamHI-BglII DNA fragment. Streptococcal pyrogenic exotoxin type B, partially purified from E. coli clones, was immunologically related to streptococcus-derived toxin. Also, toxin derived from either E. coli or Streptococcus pyogenes had similar lymphocyte mitogenic activity and molecular weight (29,300) and displayed comparable microheterogeneity when evaluated by isoelectric focusing.

Original languageEnglish (US)
Pages (from-to)1665-1667
Number of pages3
JournalInfection and immunity
Volume56
Issue number6
StatePublished - 1988

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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