Cloning, sequencing, and characterization of LDH-C4 from a fox testis cDNA library

Mark P. Bradley*, Amber Geelan, Virginia Leitch, Erwin Goldberg

*Corresponding author for this work

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

A full-length cDNA encoding the sperm-specific enzyme lactate dehydrogenase-C4 was isolated from a fox testis cDNA expression library and sequenced. The deduced translated protein sequence was shown to be 86% identical to that of human LDH-C4. In the fox testis, mRNA encoding LDH- C4 was first detected in pachytene spermatocytes. The LDH-C4 protein monomer was identified in Western blots of sperm membrane extracts as having a molecular weight of approximately 35,000, consistent with the monomeric size of this sub unit previously identified in sperm from other species. The LDH-C4 protein is localized on the sperm plasma membrane overlying the principal piece of the tail. Based on the available sequence data, we were able to identify an epitope within the N-teminal region of the LDH-C4 amino-acid sequence which when administered to female foxes is antigenic and produces antibodies capable of recognizing the native protein.

Original languageEnglish (US)
Pages (from-to)452-459
Number of pages8
JournalMolecular reproduction and development
Volume44
Issue number4
DOIs
StatePublished - Aug 1996

Keywords

  • Immunocontraception
  • LDH-C
  • Spermatozoa
  • Testis
  • Vulpes vulpes

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

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