Clustered genes required for synthesis and deposition of envelope glycolipids in Anabaena sp. strain PCC 7120

Qing Fan, Guocun Huang, Sigal Lechno-Yossef, C. Peter Wolk*, Takakazu Kaneko, Satoshi Tabata

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

81 Scopus citations

Abstract

Photoreduction of dinitrogen by heterocyst-forming cyanobacteria is of great importance ecologically and for subsistence rice agriculture. Their heterocysts must have a glycolipid envelope layer that limits the entry of oxygen if nitrogenase is to remain active to fix dinitrogen in an oxygen-containing milieu (the Fox+ phenotype). Genes alr5354 (hgID), alr5355 (hgIC) and alr5357 (hgIB) of the filamentous cyanobacterium, Anabaena sp. strain PCC 7120, and hgIE of Nostoc punctiforme are required for synthesis of heterocyst envelope glycolipids. Newly identified Fox- mutants bear transposons in nearby open reading frames (orfs) all5343, all5345-asr5349 and alr5351-alr5358. Complementation and other analysis provide evidence that at least orfs all5343 (or a co-transcribed gene), all5345, all5347, alr5348, asr5350-alr5353 and alr5356, but not asr5349, are also required for a Fox + phenotype. Lipid and sequence analyses suggest that alr5351-alr5357 encode the enzymes that biosynthesize the glycolipid aglycones. Electron microscopy indicates a role of all5345 through all5347 in the normal deposition of the envelope glycolipids.

Original languageEnglish (US)
Pages (from-to)227-243
Number of pages17
JournalMolecular Microbiology
Volume58
Issue number1
DOIs
StatePublished - Oct 2005

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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