Abstract
The novel glutamate antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) inhibited glutamate stimulated [3H]GABA release from cortical neurons in vitro. Kainate-induced release was blocked in a competitive fashion but N-methyl-d-aspartate (NMDA)-induced release was blocked non-competitively by CNQX. 7-Chlorokynurenate (7-CK) also inhibited NMDA evoked [3H]GABA release non-competitively, but had no effect on kainate induced release. The effects of both CNQX and 7-CK on NMDA-induced release were reversed by addition of exogenous glycine but the effects of CNQX on kainate-induced release were not altered by glycine. This suggests that both CNQX and 7-CK may interact with the glycine regulatory site of the NMDA receptor.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 185-189 |
| Number of pages | 5 |
| Journal | Brain research |
| Volume | 489 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jun 5 1989 |
Funding
This work was supported by U.S.P.H.S. Grants DA-02121, DA-02565, MH-40165 and grants from Miles Pharmaceuticals and Marion Labs to R.J.M., and training Grant GM-07151 to K.M.H.
Keywords
- 6-Cyano-7-nitroquinoxaline-2,3-dione
- 7-Chlorokynurenate
- Cortex
- Kainate receptor
- N-methyl-d-aspartate receptor
- γ-aminobutyric acid release
ASJC Scopus subject areas
- Clinical Neurology
- Molecular Biology
- General Neuroscience
- Developmental Biology