CNS microvascular pericytes express macrophage-like function, cell surface integrin αM, and macrophage marker ED-2

Roumen Balabanov; Ruth Washington; Jarmila Wagnerova; Paula Dore-Duffy

doi:10.1006/mvre.1996.0049

CNS microvascular pericytes express macrophage-like function, cell surface integrin αM, and macrophage marker ED-2

Roumen Balabanov, Ruth Washington, Jarmila Wagnerova, Paula Dore-Duffy^*

^*Corresponding author for this work

Neurology

Research output: Contribution to journal › Article › peer-review

144 Scopus citations

Abstract

CNS pericytes are an integral part of the blood-brain barrier (BBB), but their function is not well understood. We questioned whether primary cultured CNS pericytes have immune potential. Primary cultured pericytes exhibit phagocytic activity when exposed to fluorochrome-conjugated polystyrene beads and antibody-coated zymosan. Maximum phagocytic activity occurred by 3 hr. Pericytes were found to express the macrophage markers ED-2 and the integrin subunit CD11b (αM) in culture as well as on isolated microvessels. Pericytes did not express the macrophage marker ED-1. We confirm the heterogeneity of cultured CNS pericytes with regard to expression of α-smooth muscle actin. In conclusion, pericytes express macrophage surface antigens and have the ability to perform at least some immune function. CNS pericytes may therefore have a role in neuroimmune networks at the BBB.

Original language	English (US)
Pages (from-to)	127-142
Number of pages	16
Journal	Microvascular Research
Volume	52
Issue number	2
DOIs	https://doi.org/10.1006/mvre.1996.0049
State	Published - Sep 1996

ASJC Scopus subject areas

Biochemistry
Cardiology and Cardiovascular Medicine
Cell Biology

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title = "CNS microvascular pericytes express macrophage-like function, cell surface integrin αM, and macrophage marker ED-2",

abstract = "CNS pericytes are an integral part of the blood-brain barrier (BBB), but their function is not well understood. We questioned whether primary cultured CNS pericytes have immune potential. Primary cultured pericytes exhibit phagocytic activity when exposed to fluorochrome-conjugated polystyrene beads and antibody-coated zymosan. Maximum phagocytic activity occurred by 3 hr. Pericytes were found to express the macrophage markers ED-2 and the integrin subunit CD11b (αM) in culture as well as on isolated microvessels. Pericytes did not express the macrophage marker ED-1. We confirm the heterogeneity of cultured CNS pericytes with regard to expression of α-smooth muscle actin. In conclusion, pericytes express macrophage surface antigens and have the ability to perform at least some immune function. CNS pericytes may therefore have a role in neuroimmune networks at the BBB.",

author = "Roumen Balabanov and Ruth Washington and Jarmila Wagnerova and Paula Dore-Duffy",

note = "Funding Information: 1 Supported by Grants NS31287-04 and NS29763-03 from the National Institutes of Health. 2 To whom correspondence should be addressed.",

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T1 - CNS microvascular pericytes express macrophage-like function, cell surface integrin αM, and macrophage marker ED-2

AU - Balabanov, Roumen

AU - Washington, Ruth

AU - Wagnerova, Jarmila

AU - Dore-Duffy, Paula

N1 - Funding Information: 1 Supported by Grants NS31287-04 and NS29763-03 from the National Institutes of Health. 2 To whom correspondence should be addressed.

PY - 1996/9

Y1 - 1996/9

N2 - CNS pericytes are an integral part of the blood-brain barrier (BBB), but their function is not well understood. We questioned whether primary cultured CNS pericytes have immune potential. Primary cultured pericytes exhibit phagocytic activity when exposed to fluorochrome-conjugated polystyrene beads and antibody-coated zymosan. Maximum phagocytic activity occurred by 3 hr. Pericytes were found to express the macrophage markers ED-2 and the integrin subunit CD11b (αM) in culture as well as on isolated microvessels. Pericytes did not express the macrophage marker ED-1. We confirm the heterogeneity of cultured CNS pericytes with regard to expression of α-smooth muscle actin. In conclusion, pericytes express macrophage surface antigens and have the ability to perform at least some immune function. CNS pericytes may therefore have a role in neuroimmune networks at the BBB.

AB - CNS pericytes are an integral part of the blood-brain barrier (BBB), but their function is not well understood. We questioned whether primary cultured CNS pericytes have immune potential. Primary cultured pericytes exhibit phagocytic activity when exposed to fluorochrome-conjugated polystyrene beads and antibody-coated zymosan. Maximum phagocytic activity occurred by 3 hr. Pericytes were found to express the macrophage markers ED-2 and the integrin subunit CD11b (αM) in culture as well as on isolated microvessels. Pericytes did not express the macrophage marker ED-1. We confirm the heterogeneity of cultured CNS pericytes with regard to expression of α-smooth muscle actin. In conclusion, pericytes express macrophage surface antigens and have the ability to perform at least some immune function. CNS pericytes may therefore have a role in neuroimmune networks at the BBB.

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CNS microvascular pericytes express macrophage-like function, cell surface integrin αM, and macrophage marker ED-2

Abstract

ASJC Scopus subject areas

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