CNS myeloid DCs presenting endogenous myelin peptides 'preferentially' polarize CD4+ TH-17 cells in relapsing EAE

Samantha L. Bailey, Bettina Schreiner, Eileen J. McMahon, Stephen D. Miller*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

364 Scopus citations

Abstract

Peripherally derived CD11b+ myeloid dendritic cells (mDCs), plasmacytoid DCs, CD8α+ DCs and macrophages accumulate in the central nervous system during relapsing experimental autoimmune encephalomyelitis (EAE). During acute relapsing EAE induced by a proteolipid protein peptide of amino acids 178-191, transgenic T cells (139TCR cells) specific for the relapse epitope consisting of proteolipid protein peptide amino acids 139-151 clustered with mDCs in the central nervous system, were activated and differentiated into T helper cells producing interleukin 17 (TH-17 cells). CNS mDCs presented endogenously acquired peptide, driving the proliferation of and production of interleukin 17 by naive 139TCR cells in vitro and in vivo. The mDCs uniquely biased TH-17 and not TH1 differentiation, correlating with their enhanced expression of transforming growth factor-β1 and interleukins 6 and 23. Plasmacytoid DCs and CD8α+ DCs were superior to macrophages but were much less efficient than mDCs in presenting endogenous peptide to induce TH-17 cells. Our findings indicate a critical function for CNS mDCs in driving relapses in relapsing EAE.

Original languageEnglish (US)
Pages (from-to)172-180
Number of pages9
JournalNature Immunology
Volume8
Issue number2
DOIs
StatePublished - Feb 2007

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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