Codon recognition during frameshift suppression in Saccharomyces cerevisiae

R. F. Gaber, M. R. Culbertson

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

A genetic approach has been used to establish the molecular basis of 4-base codon recognition by frameshift suppressor tRNA containing an extra nucleotide in the anticodon. We have isolated all possible base substitution mutations at the position 4 (N) in the 3'-CCCN-5' anticodon of a Saccharomyces cerevisiae frameshift suppressor glycine tRNA encoded by the SUF16 gene. Base substitutions at +1 frameshift sites in the his4 gene have also been obtained such that all possible 4-base 5'-GGGN-3' codons have been identified. By testing for suppression in different strains that collectively represent all 16 possible combinations of position 4 nucleotides, we show that frameshift suppression does not require position 4 base paring. Nonetheless, position 4 interactions influence the efficiency of suppression. Our results suggest a model in which 4-base translocation of mRNA on the ribosome is directed primarily by the number of nucleotides in the anticodon loop, whereas the resulting efficiency of suppression is dependent on the nature of position 4 nucleotides.

Original languageEnglish (US)
Pages (from-to)2052-2061
Number of pages10
JournalMolecular and cellular biology
Volume4
Issue number10
DOIs
StatePublished - 1984

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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