Coexpression of glutamine synthetase and carbamoylphosphate synthase I genes in pancreatic hepatocytes of rat

A. V. Yeldandi*, X. Tan, R. S. Dwivedi, V. Subbarao, D. D. Smith, D. G. Scarpelli, M. S. Rao, J. K. Reddy

*Corresponding author for this work

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

In the mammalian liver the distribution of ammonia-detoxifying enzymes, glutamine synthetase (GS) and carbamoylphosphate synthase I (ammonia) (CPS-I), is mutually exclusive in that these enzymes are expressed in two distinct populations of hepatocytes that are zonally demarcated in the liver acinus. In the present study we examined the distribution of GS and CPS-I in pancreatic hepatocytes to ascertain if the expression of these two genes in these hepatocytes is also mutually exclusive. Multiple foci of hepatocytes showing no clear acinar organization develop in the adult rat pancreas as a result of a change in the differentiation commitment after dietary copper deficiency. Unlike liver, GS and CPS-I are detected by immunofluorescence in all pancreatic hepatocytes. In situ hybridization revealed that all pancreatic hepatocytes contain GS and CPS-I mRNAs. The sizes of these two mRNAs in pancreas with hepatocytes are similar to those of the liver. The concomitant expression of GS and CPS-I genes in pancreatic hepatocytes may be attributed, in part, to the absence of portal blood supply to the pancreas vis-a-vis the lack of hormonal/metabolic gradients as well as to possible matrix homogeneity in the pancreas.

Original languageEnglish (US)
Pages (from-to)881-885
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number3
DOIs
StatePublished - 1990

Keywords

  • Differentiation
  • in situ hybridization
  • liver parenchymal cells

ASJC Scopus subject areas

  • General

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