Abstract
Treatment of cultured mouse embryo fibroblasts with Triton X-100 after prolonged incubation at 0 °C reveals a network of microtubules in the cytoplasm of cooled cells. This network of cold-stable microtubules was demonstrated by immunofluorescence microscopy, using a monospecific antibody against tubulin and by electron microscopy. The cold-stable microtubules, as well as the ordinary cytoplasmic microtubules, were sensitive to Ca ions and were not observed in the cells pre-treated with colchicine or vinblastine. The cold-stable microtubules do not seem to be in equilibrium with the pool of depolymerized tubulin at 0 °C.
Original language | English (US) |
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Pages (from-to) | 45-50 |
Number of pages | 6 |
Journal | Cell Biology International Reports |
Volume | 3 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1979 |
ASJC Scopus subject areas
- Cell Biology