Cold-stable microtubules in the cytoplasm of mouse embryo fibroblasts

A. D. Bershadsky; V. I. Gelfand; T. M. Svitkina; I. S. Tint

doi:10.1016/0309-1651(79)90067-5

Cold-stable microtubules in the cytoplasm of mouse embryo fibroblasts

A. D. Bershadsky, V. I. Gelfand^*, T. M. Svitkina, I. S. Tint

^*Corresponding author for this work

Cell & Developmental Biology

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Abstract

Treatment of cultured mouse embryo fibroblasts with Triton X-100 after prolonged incubation at 0 °C reveals a network of microtubules in the cytoplasm of cooled cells. This network of cold-stable microtubules was demonstrated by immunofluorescence microscopy, using a monospecific antibody against tubulin and by electron microscopy. The cold-stable microtubules, as well as the ordinary cytoplasmic microtubules, were sensitive to Ca ions and were not observed in the cells pre-treated with colchicine or vinblastine. The cold-stable microtubules do not seem to be in equilibrium with the pool of depolymerized tubulin at 0 °C.

Original language	English (US)
Pages (from-to)	45-50
Number of pages	6
Journal	Cell Biology International Reports
Volume	3
Issue number	1
DOIs	https://doi.org/10.1016/0309-1651(79)90067-5
State	Published - Jan 1979

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Cell Biology

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10.1016/0309-1651(79)90067-5

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abstract = "Treatment of cultured mouse embryo fibroblasts with Triton X-100 after prolonged incubation at 0 °C reveals a network of microtubules in the cytoplasm of cooled cells. This network of cold-stable microtubules was demonstrated by immunofluorescence microscopy, using a monospecific antibody against tubulin and by electron microscopy. The cold-stable microtubules, as well as the ordinary cytoplasmic microtubules, were sensitive to Ca ions and were not observed in the cells pre-treated with colchicine or vinblastine. The cold-stable microtubules do not seem to be in equilibrium with the pool of depolymerized tubulin at 0 °C.",

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AU - Gelfand, V. I.

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AU - Tint, I. S.

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AB - Treatment of cultured mouse embryo fibroblasts with Triton X-100 after prolonged incubation at 0 °C reveals a network of microtubules in the cytoplasm of cooled cells. This network of cold-stable microtubules was demonstrated by immunofluorescence microscopy, using a monospecific antibody against tubulin and by electron microscopy. The cold-stable microtubules, as well as the ordinary cytoplasmic microtubules, were sensitive to Ca ions and were not observed in the cells pre-treated with colchicine or vinblastine. The cold-stable microtubules do not seem to be in equilibrium with the pool of depolymerized tubulin at 0 °C.

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Cold-stable microtubules in the cytoplasm of mouse embryo fibroblasts

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