Abstract
Treatment of cultured mouse embryo fibroblasts with Triton X-100 after prolonged incubation at 0 °C reveals a network of microtubules in the cytoplasm of cooled cells. This network of cold-stable microtubules was demonstrated by immunofluorescence microscopy, using a monospecific antibody against tubulin and by electron microscopy. The cold-stable microtubules, as well as the ordinary cytoplasmic microtubules, were sensitive to Ca ions and were not observed in the cells pre-treated with colchicine or vinblastine. The cold-stable microtubules do not seem to be in equilibrium with the pool of depolymerized tubulin at 0 °C.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 45-50 |
| Number of pages | 6 |
| Journal | Cell Biology International Reports |
| Volume | 3 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 1979 |
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- Cell Biology
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Cold-stable microtubules in the cytoplasm of mouse embryo fibroblasts. / Bershadsky, A. D.; Gelfand, V. I.; Svitkina, T. M.; Tint, I. S.
In: Cell Biology International Reports, Vol. 3, No. 1, 01.01.1979, p. 45-50.Research output: Contribution to journal › Article
TY - JOUR
T1 - Cold-stable microtubules in the cytoplasm of mouse embryo fibroblasts
AU - Bershadsky, A. D.
AU - Gelfand, V. I.
AU - Svitkina, T. M.
AU - Tint, I. S.
PY - 1979/1/1
Y1 - 1979/1/1
N2 - Treatment of cultured mouse embryo fibroblasts with Triton X-100 after prolonged incubation at 0 °C reveals a network of microtubules in the cytoplasm of cooled cells. This network of cold-stable microtubules was demonstrated by immunofluorescence microscopy, using a monospecific antibody against tubulin and by electron microscopy. The cold-stable microtubules, as well as the ordinary cytoplasmic microtubules, were sensitive to Ca ions and were not observed in the cells pre-treated with colchicine or vinblastine. The cold-stable microtubules do not seem to be in equilibrium with the pool of depolymerized tubulin at 0 °C.
AB - Treatment of cultured mouse embryo fibroblasts with Triton X-100 after prolonged incubation at 0 °C reveals a network of microtubules in the cytoplasm of cooled cells. This network of cold-stable microtubules was demonstrated by immunofluorescence microscopy, using a monospecific antibody against tubulin and by electron microscopy. The cold-stable microtubules, as well as the ordinary cytoplasmic microtubules, were sensitive to Ca ions and were not observed in the cells pre-treated with colchicine or vinblastine. The cold-stable microtubules do not seem to be in equilibrium with the pool of depolymerized tubulin at 0 °C.
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UR - http://www.scopus.com/inward/citedby.url?scp=0018414159&partnerID=8YFLogxK
U2 - 10.1016/0309-1651(79)90067-5
DO - 10.1016/0309-1651(79)90067-5
M3 - Article
VL - 3
SP - 45
EP - 50
JO - Cell Biology International
JF - Cell Biology International
SN - 1065-6995
IS - 1
ER -