Cold-stable microtubules in the cytoplasm of mouse embryo fibroblasts

A. D. Bershadsky; V. I. Gelfand; T. M. Svitkina; I. S. Tint

doi:10.1016/0309-1651(79)90067-5

Cold-stable microtubules in the cytoplasm of mouse embryo fibroblasts

A. D. Bershadsky, V. I. Gelfand^*, T. M. Svitkina, I. S. Tint

^*Corresponding author for this work

Cell & Developmental Biology

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Treatment of cultured mouse embryo fibroblasts with Triton X-100 after prolonged incubation at 0 °C reveals a network of microtubules in the cytoplasm of cooled cells. This network of cold-stable microtubules was demonstrated by immunofluorescence microscopy, using a monospecific antibody against tubulin and by electron microscopy. The cold-stable microtubules, as well as the ordinary cytoplasmic microtubules, were sensitive to Ca ions and were not observed in the cells pre-treated with colchicine or vinblastine. The cold-stable microtubules do not seem to be in equilibrium with the pool of depolymerized tubulin at 0 °C.

Original language	English (US)
Pages (from-to)	45-50
Number of pages	6
Journal	Cell Biology International Reports
Volume	3
Issue number	1
DOIs	https://doi.org/10.1016/0309-1651(79)90067-5
State	Published - Jan 1979

ASJC Scopus subject areas

Cell Biology

Access to Document

10.1016/0309-1651(79)90067-5

Fingerprint Dive into the research topics of 'Cold-stable microtubules in the cytoplasm of mouse embryo fibroblasts'. Together they form a unique fingerprint.

Cite this

@article{6fcae9b16fa24a3e8157dfb4663e047c,

title = "Cold-stable microtubules in the cytoplasm of mouse embryo fibroblasts",

abstract = "Treatment of cultured mouse embryo fibroblasts with Triton X-100 after prolonged incubation at 0 °C reveals a network of microtubules in the cytoplasm of cooled cells. This network of cold-stable microtubules was demonstrated by immunofluorescence microscopy, using a monospecific antibody against tubulin and by electron microscopy. The cold-stable microtubules, as well as the ordinary cytoplasmic microtubules, were sensitive to Ca ions and were not observed in the cells pre-treated with colchicine or vinblastine. The cold-stable microtubules do not seem to be in equilibrium with the pool of depolymerized tubulin at 0 °C.",

author = "Bershadsky, {A. D.} and Gelfand, {V. I.} and Svitkina, {T. M.} and Tint, {I. S.}",

year = "1979",

month = jan,

doi = "10.1016/0309-1651(79)90067-5",

language = "English (US)",

volume = "3",

pages = "45--50",

journal = "Cell Biology International",

issn = "1065-6995",

publisher = "Portland Press Ltd.",

number = "1",

}

TY - JOUR

T1 - Cold-stable microtubules in the cytoplasm of mouse embryo fibroblasts

AU - Bershadsky, A. D.

AU - Gelfand, V. I.

AU - Svitkina, T. M.

AU - Tint, I. S.

PY - 1979/1

Y1 - 1979/1

N2 - Treatment of cultured mouse embryo fibroblasts with Triton X-100 after prolonged incubation at 0 °C reveals a network of microtubules in the cytoplasm of cooled cells. This network of cold-stable microtubules was demonstrated by immunofluorescence microscopy, using a monospecific antibody against tubulin and by electron microscopy. The cold-stable microtubules, as well as the ordinary cytoplasmic microtubules, were sensitive to Ca ions and were not observed in the cells pre-treated with colchicine or vinblastine. The cold-stable microtubules do not seem to be in equilibrium with the pool of depolymerized tubulin at 0 °C.

AB - Treatment of cultured mouse embryo fibroblasts with Triton X-100 after prolonged incubation at 0 °C reveals a network of microtubules in the cytoplasm of cooled cells. This network of cold-stable microtubules was demonstrated by immunofluorescence microscopy, using a monospecific antibody against tubulin and by electron microscopy. The cold-stable microtubules, as well as the ordinary cytoplasmic microtubules, were sensitive to Ca ions and were not observed in the cells pre-treated with colchicine or vinblastine. The cold-stable microtubules do not seem to be in equilibrium with the pool of depolymerized tubulin at 0 °C.

UR - http://www.scopus.com/inward/record.url?scp=0018414159&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0018414159&partnerID=8YFLogxK

U2 - 10.1016/0309-1651(79)90067-5

DO - 10.1016/0309-1651(79)90067-5

M3 - Article

C2 - 378425

AN - SCOPUS:0018414159

VL - 3

SP - 45

EP - 50

JO - Cell Biology International

JF - Cell Biology International

SN - 1065-6995

IS - 1

ER -

Cold-stable microtubules in the cytoplasm of mouse embryo fibroblasts

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this