Cold-stable microtubules in the cytoplasm of mouse embryo fibroblasts

A. D. Bershadsky, V. I. Gelfand*, T. M. Svitkina, I. S. Tint

*Corresponding author for this work

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Treatment of cultured mouse embryo fibroblasts with Triton X-100 after prolonged incubation at 0 °C reveals a network of microtubules in the cytoplasm of cooled cells. This network of cold-stable microtubules was demonstrated by immunofluorescence microscopy, using a monospecific antibody against tubulin and by electron microscopy. The cold-stable microtubules, as well as the ordinary cytoplasmic microtubules, were sensitive to Ca ions and were not observed in the cells pre-treated with colchicine or vinblastine. The cold-stable microtubules do not seem to be in equilibrium with the pool of depolymerized tubulin at 0 °C.

Original languageEnglish (US)
Pages (from-to)45-50
Number of pages6
JournalCell Biology International Reports
Volume3
Issue number1
DOIs
StatePublished - Jan 1 1979

ASJC Scopus subject areas

  • Cell Biology

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