Colorimetric Cu2+ detection using DNA-modified gold-nanoparticle aggregates as probes and click chemistry

Xiaoyang Xu; Weston L. Daniel; Wei Wei; Chad A. Mirkin

doi:10.1002/smll.200901691

Colorimetric Cu²⁺ detection using DNA-modified gold-nanoparticle aggregates as probes and click chemistry

Xiaoyang Xu^*, Weston L. Daniel, Wei Wei, Chad A. Mirkin

^*Corresponding author for this work

Chemistry

Research output: Contribution to journal › Article › peer-review

248 Scopus citations

Abstract

The colorimetric detection of Cu²⁺ is demonstrated using DNA-modified gold nanoparticle (Au NP) aggregates as probes and a copper-catalyzed click reaction to initiate the cross-coupling of alkynes and azides that terminate the DNA on the particles. The ligation of two oligonucleotides via click chemistry and copper ions raises the melting temperature of that duplex, which can be monitored colorimetrically using the optical properties of the Au NPs.

Original language	English (US)
Pages (from-to)	623-626
Number of pages	4
Journal	Small
Volume	6
Issue number	5
DOIs	https://doi.org/10.1002/smll.200901691
State	Published - Mar 8 2010

Keywords

Click chemistry
Colorimetric detection
Copper
DNA
Nanoparticle conjugates

ASJC Scopus subject areas

General Chemistry
General Materials Science
Biotechnology
Biomaterials

Access to Document

10.1002/smll.200901691

Cite this

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abstract = "The colorimetric detection of Cu2+ is demonstrated using DNA-modified gold nanoparticle (Au NP) aggregates as probes and a copper-catalyzed click reaction to initiate the cross-coupling of alkynes and azides that terminate the DNA on the particles. The ligation of two oligonucleotides via click chemistry and copper ions raises the melting temperature of that duplex, which can be monitored colorimetrically using the optical properties of the Au NPs.",

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AU - Daniel, Weston L.

AU - Wei, Wei

AU - Mirkin, Chad A.

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KW - Colorimetric detection

KW - Copper

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KW - Nanoparticle conjugates

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