@inbook{e63145b524374ac7ac95433de8f82c40,
title = "Combinatorial screening of mesenchymal stem cell adhesion and differentiation using polymer pen lithography",
abstract = "The extracellular matrix (ECM) is a complex, spatially inhomogeneous environment that is host to myriad cell-receptor interactions that promote changes in cell behavior. These biological systems can be probed and simulated with engineered surfaces, but doing so demands careful control over the arrangement of ligands. Here, we describe how such surfaces can be fabricated by utilizing polymer pen lithography (PPL), which is a cantilever-free scanning probe lithographic method that utilizes polymeric pen arrays to generate patterns over large areas. With the advent of PPL, fundamental questions in cell biology can be answered by recapitulating cell-ECM interactions to explore how these interactions lead to changes in cell behavior. Here, we describe an approach for the combinatorial screening of cell adhesion behavior to gain understanding of how ECM protein feature size dictates osteogenic differentiation of mesenchymal stem cells. The technique outlined here is generalizable to other biological systems and can be paired with quantitative analytical methods to probe important processes such as cell polarization, proliferation, signaling, and differentiation.",
keywords = "Cell adhesion, Nanopatterning, Osteogenesis, Polymer pen lithography, Stem cell differentiation",
author = "Cabezas, {Maria D.} and Eichelsdoerfer, {Daniel J.} and Brown, {Keith A.} and Milan Mrksich and Mirkin, {Chad A.}",
note = "Funding Information: This material is based upon work supported by the Air Force Office of Scientific Research (AFOSR), under award number FA9550-08-1-0124, SPAWAR Systems Center Pacific Code 22550 under award number N66001-08-1-2044, the National Cancer Institute under award number U54CA151880, the National Science Foundation (NSF) Nanoscale Sciences and Engineering Initiative under award number EEC-0647560, and the Defense Advanced Research Projects Agency (DARPA). D.J.E. is supported by a DoD, Air Force Office of Scientific Research, National Defense Science and Engineering Graduate (NDSEG) Fellowship, 32 CFR 168a. K.A.B. gratefully acknowledges support from Northwestern University{\textquoteright}s International Institute for Nanotechnology. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.",
year = "2014",
doi = "10.1016/B978-0-12-416742-1.00013-5",
language = "English (US)",
series = "Methods in Cell Biology",
publisher = "Academic Press Inc",
pages = "261--276",
booktitle = "Methods in Cell Biology",
}