TY - JOUR
T1 - Combined Biomaterials
T2 - Amniotic Membrane and Adipose Tissue to Restore Injured Bone as Promoter of Calcification in Bone Regeneration: Preclinical Model
AU - Dziedzic, Dilcele Silva Moreira
AU - Francisco, Júlio César
AU - Mogharbel, Bassam Felipe
AU - Irioda, Ana Carolina
AU - Stricker, Priscila Elias Ferreira
AU - Floriano, Juliana
AU - de Noronha, Lúcia
AU - Abdelwahid, Eltyeb
AU - Franco, Célia Regina Cavichiolo
AU - de Carvalho, Katherine Athayde Teixeira
N1 - Funding Information:
This study was supported by Pelé Pequeno Príncipe Institute, Child and Adolescent Health Research (State of Paraná- Brazil), and CAPES- Brazil (Coordination for the Improvement of Higher Education Personnel) provided students’ scholarships (Finance Code 001).
Publisher Copyright:
© 2021, The Author(s).
PY - 2021/5
Y1 - 2021/5
N2 - Discarded tissues, like human amniotic membranes and adipose tissue, were investigated for the application of Decellularized Human Amniotic Membrane (DAM) as a viable scaffold for transplantation of Adipose-derived stromal cells (ASCs) in bone regeneration of non-healing calvarial defects in rats. Amniotic membrane was decellularized to provide a scaffold for male Wistar rats ASCs expansion and transplantation. ASCs osteoinduction in vitro promoted the deposition of a mineralized bone-like matrix by ASCs, as calcified globular accretions associated with the cells on the DAM surface and inside the collagenous matrix. Non-healing calvarial defects on male Wistar rats were randomly divided in control without treatment, treatment with four layers of DAM, or four layers of DAM associated with ASCs. After 12 weeks, tissue blocks were examined by micro-computed tomography and histology. DAM promoted osteoconduction by increasing the collagenous matrix on both DAM treatments. DAM with ASCs stimulated bone deposition, demonstrated by a higher percentage of bone volume and trabecular bone number, compared to control. Besides the osteogenic capacity in vitro, ASCs stimulated the healing of calvarial defects with significant DAM graft incorporation concomitant with higher host bone deposition. The enhanced in vivo bone regeneration by undifferentiated ASCs loaded onto DAM confirmed the potential of an easily collected autologous cell source associated with a broadly available collagenous matrix in tissue engineering.
AB - Discarded tissues, like human amniotic membranes and adipose tissue, were investigated for the application of Decellularized Human Amniotic Membrane (DAM) as a viable scaffold for transplantation of Adipose-derived stromal cells (ASCs) in bone regeneration of non-healing calvarial defects in rats. Amniotic membrane was decellularized to provide a scaffold for male Wistar rats ASCs expansion and transplantation. ASCs osteoinduction in vitro promoted the deposition of a mineralized bone-like matrix by ASCs, as calcified globular accretions associated with the cells on the DAM surface and inside the collagenous matrix. Non-healing calvarial defects on male Wistar rats were randomly divided in control without treatment, treatment with four layers of DAM, or four layers of DAM associated with ASCs. After 12 weeks, tissue blocks were examined by micro-computed tomography and histology. DAM promoted osteoconduction by increasing the collagenous matrix on both DAM treatments. DAM with ASCs stimulated bone deposition, demonstrated by a higher percentage of bone volume and trabecular bone number, compared to control. Besides the osteogenic capacity in vitro, ASCs stimulated the healing of calvarial defects with significant DAM graft incorporation concomitant with higher host bone deposition. The enhanced in vivo bone regeneration by undifferentiated ASCs loaded onto DAM confirmed the potential of an easily collected autologous cell source associated with a broadly available collagenous matrix in tissue engineering.
KW - Adipose stem cells
KW - Bone tissue engineering
KW - Human amniotic membrane
KW - Stem cell transplantation
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U2 - 10.1007/s00223-020-00793-1
DO - 10.1007/s00223-020-00793-1
M3 - Article
C2 - 33420810
AN - SCOPUS:85098973559
SN - 0171-967X
VL - 108
SP - 667
EP - 679
JO - Calcified Tissue International
JF - Calcified Tissue International
IS - 5
ER -