Combined photoacoustic microscopy and optical coherence tomography can measure metabolic rate of oxygen

Tan Liu, Qing Wei, Jing Wang, Shuliang Jiao*, Hao F. Zhang

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

63 Scopus citations

Abstract

We proposed to measure the metabolic rate of oxygen (MRO2) in small animals in vivo using a multimodal imaging system that combines laser-scanning optical-resolution photoacoustic microscopy (LSOR-PAM) and spectral-domain optical coherence tomography (SD-OCT). We first tested the capability of the multimodal system to measure flow rate in a phantom made of two capillary tubes of different diameters. We then demonstrated the capability of measuring MRO2 by imaging two parallel vessels selected from the ear of a Swiss Webster mouse. The hemoglobin oxygen saturation (sO2) and the vessel diameter were measured by the LSOR-PAM and the blood flow velocity was measured by the SD-OCT, from which blood flow rate and MRO2 were further calculated. The measured blood flow rates in the two vessels agreed with each other.

Original languageEnglish (US)
Pages (from-to)1359-1365
Number of pages7
JournalBiomedical Optics Express
Volume2
Issue number5
DOIs
StatePublished - May 1 2011

ASJC Scopus subject areas

  • Biotechnology
  • Atomic and Molecular Physics, and Optics

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