Combined tyramide signal amplification and quantum dots for sensitive and photostable immunofluorescence detection

Jayne M. Ness, Rizwan S. Akhtar, Cecelia B. Latham, Kevin A. Roth*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

80 Scopus citations

Abstract

Conventional immunofluorescence detection of biologically relevant proteins and antigens in tissue sections is often limited by relatively weak signals that fade rapidly on illumination. We have developed an immunohistochemical protocol that combines the sensitivity of tyramide signal amplification with the photostability of quantum dots to overcome these limitations. This simple method provides a sensitive and stable fluorescence immunohistochemical alternative to standard chromogen detection.

Original languageEnglish (US)
Pages (from-to)981-987
Number of pages7
JournalJournal of Histochemistry and Cytochemistry
Volume51
Issue number8
DOIs
StatePublished - Aug 1 2003
Externally publishedYes

Keywords

  • Immunohistochemistry
  • Nanocrystals
  • Photobleaching
  • Tyramide signal amplification

ASJC Scopus subject areas

  • Anatomy
  • Histology

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