Combined tyramide signal amplification and quantum dots for sensitive and photostable immunofluorescence detection

Jayne M. Ness; Rizwan S. Akhtar; Cecelia B. Latham; Kevin A. Roth

doi:10.1177/002215540305100801

Combined tyramide signal amplification and quantum dots for sensitive and photostable immunofluorescence detection

Jayne M. Ness, Rizwan S. Akhtar, Cecelia B. Latham, Kevin A. Roth^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

89 Scopus citations

Abstract

Conventional immunofluorescence detection of biologically relevant proteins and antigens in tissue sections is often limited by relatively weak signals that fade rapidly on illumination. We have developed an immunohistochemical protocol that combines the sensitivity of tyramide signal amplification with the photostability of quantum dots to overcome these limitations. This simple method provides a sensitive and stable fluorescence immunohistochemical alternative to standard chromogen detection.

Original language	English (US)
Pages (from-to)	981-987
Number of pages	7
Journal	Journal of Histochemistry and Cytochemistry
Volume	51
Issue number	8
DOIs	https://doi.org/10.1177/002215540305100801
State	Published - Aug 1 2003
Externally published	Yes

Funding

Keywords

Immunohistochemistry
Nanocrystals
Photobleaching
Tyramide signal amplification

ASJC Scopus subject areas

Anatomy
Histology

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10.1177/002215540305100801

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title = "Combined tyramide signal amplification and quantum dots for sensitive and photostable immunofluorescence detection",

abstract = "Conventional immunofluorescence detection of biologically relevant proteins and antigens in tissue sections is often limited by relatively weak signals that fade rapidly on illumination. We have developed an immunohistochemical protocol that combines the sensitivity of tyramide signal amplification with the photostability of quantum dots to overcome these limitations. This simple method provides a sensitive and stable fluorescence immunohistochemical alternative to standard chromogen detection.",

keywords = "Immunohistochemistry, Nanocrystals, Photobleaching, Tyramide signal amplification",

author = "Ness, {Jayne M.} and Akhtar, {Rizwan S.} and Latham, {Cecelia B.} and Roth, {Kevin A.}",

year = "2003",

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doi = "10.1177/002215540305100801",

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T1 - Combined tyramide signal amplification and quantum dots for sensitive and photostable immunofluorescence detection

AU - Ness, Jayne M.

AU - Akhtar, Rizwan S.

AU - Latham, Cecelia B.

AU - Roth, Kevin A.

PY - 2003/8/1

Y1 - 2003/8/1

N2 - Conventional immunofluorescence detection of biologically relevant proteins and antigens in tissue sections is often limited by relatively weak signals that fade rapidly on illumination. We have developed an immunohistochemical protocol that combines the sensitivity of tyramide signal amplification with the photostability of quantum dots to overcome these limitations. This simple method provides a sensitive and stable fluorescence immunohistochemical alternative to standard chromogen detection.

AB - Conventional immunofluorescence detection of biologically relevant proteins and antigens in tissue sections is often limited by relatively weak signals that fade rapidly on illumination. We have developed an immunohistochemical protocol that combines the sensitivity of tyramide signal amplification with the photostability of quantum dots to overcome these limitations. This simple method provides a sensitive and stable fluorescence immunohistochemical alternative to standard chromogen detection.

KW - Immunohistochemistry

KW - Nanocrystals

KW - Photobleaching

KW - Tyramide signal amplification

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M3 - Article

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JO - Journal of Histochemistry and Cytochemistry

JF - Journal of Histochemistry and Cytochemistry

IS - 8

ER -

Combined tyramide signal amplification and quantum dots for sensitive and photostable immunofluorescence detection

Abstract

Funding

Keywords

ASJC Scopus subject areas

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