Combining electron crystallography and X-ray crystallography to study the MlotiK1 cyclic nucleotide-regulated potassium channel

Gina M. Clayton*, Steve G. Aller, Jimin Wang, Vinzenz Unger, João H. Morais-Cabral

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

We have recently reported the X-ray structure of the cyclic nucleotide-regulated potassium channel, MlotiK1. Here we describe the application of both electron and X-ray crystallography to obtain high quality crystals. We suggest that the combined application of these techniques provides a useful strategy for membrane protein structure determination. We also present negative stain projection and cryo-data projection maps. These maps provide new insights about the properties of the MlotiK1 channel. In particular, a comparison of a 9 Å cryo-data projection with calculated model maps strongly suggests that there is a very weak interaction between the pore and the S1-S4 domains of this 6 TM tetrameric cation channel and that the S1-S4 domains can adopt multiple orientations relative to the pore.

Original languageEnglish (US)
Pages (from-to)220-226
Number of pages7
JournalJournal of Structural Biology
Volume167
Issue number3
DOIs
StatePublished - Sep 2009

Keywords

  • 6 transmembrane helix channel
  • Crystallization conditions
  • Cyclic nucleotide-regulated potassium channel
  • Electron crystallography
  • Membrane protein
  • X-ray crystallography

ASJC Scopus subject areas

  • Structural Biology

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