TY - JOUR
T1 - Common Regulatory Targets of NFIA, NFIX and NFIB during Postnatal Cerebellar Development
AU - Fraser, James
AU - Essebier, Alexandra
AU - Brown, Alexander S.
AU - Davila, Raul Ayala
AU - Harkins, Danyon
AU - Zalucki, Oressia
AU - Shapiro, Lauren P.
AU - Penzes, Peter
AU - Wainwright, Brandon J.
AU - Scott, Matthew P.
AU - Gronostajski, Richard M.
AU - Bodén, Mikael
AU - Piper, Michael
AU - Harvey, Tracey J.
N1 - Funding Information:
This work was supported by the Australian Research Council grants (DP160100368 and DP180100017 to MP) and NYSTEM grants (CO30133 and C30290GG to RMG). JF and AE were supported by the Research Training Program scholarships from the Australian Government. ASB was supported by Ruth L. Kirschstein (NRSA F32 GM105227).
Publisher Copyright:
© 2019, Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2020/2/1
Y1 - 2020/2/1
N2 - Transcriptional regulation plays a central role in controlling neural stem and progenitor cell proliferation and differentiation during neurogenesis. For instance, transcription factors from the nuclear factor I (NFI) family have been shown to co-ordinate neural stem and progenitor cell differentiation within multiple regions of the embryonic nervous system, including the neocortex, hippocampus, spinal cord and cerebellum. Knockout of individual Nfi genes culminates in similar phenotypes, suggestive of common target genes for these transcription factors. However, whether or not the NFI family regulates common suites of genes remains poorly defined. Here, we use granule neuron precursors (GNPs) of the postnatal murine cerebellum as a model system to analyse regulatory targets of three members of the NFI family: NFIA, NFIB and NFIX. By integrating transcriptomic profiling (RNA-seq) of Nfia- and Nfix-deficient GNPs with epigenomic profiling (ChIP-seq against NFIA, NFIB and NFIX, and DNase I hypersensitivity assays), we reveal that these transcription factors share a large set of potential transcriptional targets, suggestive of complementary roles for these NFI family members in promoting neural development.
AB - Transcriptional regulation plays a central role in controlling neural stem and progenitor cell proliferation and differentiation during neurogenesis. For instance, transcription factors from the nuclear factor I (NFI) family have been shown to co-ordinate neural stem and progenitor cell differentiation within multiple regions of the embryonic nervous system, including the neocortex, hippocampus, spinal cord and cerebellum. Knockout of individual Nfi genes culminates in similar phenotypes, suggestive of common target genes for these transcription factors. However, whether or not the NFI family regulates common suites of genes remains poorly defined. Here, we use granule neuron precursors (GNPs) of the postnatal murine cerebellum as a model system to analyse regulatory targets of three members of the NFI family: NFIA, NFIB and NFIX. By integrating transcriptomic profiling (RNA-seq) of Nfia- and Nfix-deficient GNPs with epigenomic profiling (ChIP-seq against NFIA, NFIB and NFIX, and DNase I hypersensitivity assays), we reveal that these transcription factors share a large set of potential transcriptional targets, suggestive of complementary roles for these NFI family members in promoting neural development.
KW - Cerebellum
KW - External granular layer
KW - Granule neuron
KW - NFIA
KW - NFIB
KW - NFIX
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U2 - 10.1007/s12311-019-01089-3
DO - 10.1007/s12311-019-01089-3
M3 - Article
C2 - 31838646
AN - SCOPUS:85076519863
SN - 1473-4222
VL - 19
SP - 89
EP - 101
JO - Cerebellum
JF - Cerebellum
IS - 1
ER -