Comparative analysis of flow cytometric techniques in assessment of ZAP-70 expression in relation to IgVH mutational status in chronic lymphocytic leukemia

Yi Hua Chen, Lo Ann C Peterson, David Dittmann, Andrew Evens, Steven Rosen, Adela Khoong, T. Vincent Shankey, Meryl Forman, Rohit Gupta, Charles L. Goolsby*

*Corresponding author for this work

Research output: Contribution to journalArticle

27 Scopus citations

Abstract

We compared 1 subjective and 5 objective flow cytometric methods to evaluate ζ-associated protein (ZAP-70) expression in relation to immunoglobulin heavy-chain variable-region (IgVH) gene mutational status in 154 samples from 125 patients with chronic lymphocytic leukemia (CLL). ZAP-70 expression determined by all methods used correlated with IgV H gene mutational status, but none of them demonstrated high concordance rates. Of the objective methods, ZAP-70 staining determined as a ratio of molecules of equivalent soluble fluorochrome intensity in CLL cells to that in normal B cells (ZAP-70+ staining in IgVH germline cases, 59%; ZAP-70- in IgVH mutated cases, 75%) or T cells (ZAP-70+ in IgV H germline cases, 66%; ZAP-70- in IgVH mutated cases, 57%) provides the best combination for assigning ZAP-70+ status to IgVH germline and ZAP-70- status to IgVH mutated cases. The subjective method based on ZAP-70 expression in natural killer/T cells gave a similar result, but reproducibility between laboratories may be difficult. Further studies on ZAP-70 expression in relation to clinical parameters may address whether ZAP-70 is an independent prognostic marker for CLL.

Original languageEnglish (US)
Pages (from-to)182-191
Number of pages10
JournalAmerican journal of clinical pathology
Volume127
Issue number2
DOIs
StatePublished - Feb 1 2007

Keywords

  • Chronic lymphocytic leukemia
  • Flow cytometry
  • IgV gene
  • ZAP-70

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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