Comparative gene mapping in cattle, Indian muntjac, and Chinese muntjac by fluorescence in situ hybridization

Andrea E. Murmann, Antoaneta Mincheva, Markus O. Scheuermann, Mathieu Gautier, Fentang Yang, Johannes Buitkamp, Pamela L. Strissel, Reiner Strick, Janet D. Rowley, Peter Lichter

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

The Indian muntjac (Muntiacus muntjak vaginalis) has a karyotype of 2n = 6 in the female and 2n = 7 in the male. The karyotypic evolution of Indian muntjac via extensive tandem fusions and several centric fusions are well documented by molecular cytogenetic studies mainly utilizing chromosome paints. To achieve higher resolution mapping, a set of 42 different genomic clones coding for 37 genes and the nucleolar organizer region were used to examine homologies between the cattle (2n = 60), human (2n = 46), Indian muntjac (2n = 6/7) and Chinese muntjac (2n = 46) karyotypes. These genomic clones were mapped by fluorescence in situ hybridization (FISH). Localization of genes on all three pairs of M. m. vaginalis chromosomes and on the acrocentric chromosomes of M. reevesi allowed not only the analysis of the evolution of syntenic regions within the muntjac genus but also allowed a broader comparison of synteny with more distantly related species, such as cattle and human, to shed more light onto the evolving genome organization.

Original languageEnglish (US)
Pages (from-to)345-351
Number of pages7
JournalGenetica
Volume134
Issue number3
DOIs
StatePublished - Nov 2008

Keywords

  • Bos taurus
  • Homo sapiens
  • Mammals
  • Muntiacus muntjak
  • Muntiacus reevesi
  • NOR
  • Synteny

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Genetics
  • Plant Science
  • Insect Science

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