Comparative gene mapping in cattle, Indian muntjac, and Chinese muntjac by fluorescence in situ hybridization

Andrea E. Murmann; Antoaneta Mincheva; Markus O. Scheuermann; Mathieu Gautier; Fentang Yang; Johannes Buitkamp; Pamela L. Strissel; Reiner Strick; Janet D. Rowley; Peter Lichter

doi:10.1007/s10709-008-9242-1

Comparative gene mapping in cattle, Indian muntjac, and Chinese muntjac by fluorescence in situ hybridization

Andrea E. Murmann, Antoaneta Mincheva, Markus O. Scheuermann, Mathieu Gautier, Fentang Yang, Johannes Buitkamp, Pamela L. Strissel, Reiner Strick, Janet D. Rowley, Peter Lichter

Medicine, Hematology Oncology Division

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The Indian muntjac (Muntiacus muntjak vaginalis) has a karyotype of 2n = 6 in the female and 2n = 7 in the male. The karyotypic evolution of Indian muntjac via extensive tandem fusions and several centric fusions are well documented by molecular cytogenetic studies mainly utilizing chromosome paints. To achieve higher resolution mapping, a set of 42 different genomic clones coding for 37 genes and the nucleolar organizer region were used to examine homologies between the cattle (2n = 60), human (2n = 46), Indian muntjac (2n = 6/7) and Chinese muntjac (2n = 46) karyotypes. These genomic clones were mapped by fluorescence in situ hybridization (FISH). Localization of genes on all three pairs of M. m. vaginalis chromosomes and on the acrocentric chromosomes of M. reevesi allowed not only the analysis of the evolution of syntenic regions within the muntjac genus but also allowed a broader comparison of synteny with more distantly related species, such as cattle and human, to shed more light onto the evolving genome organization.

Original language	English (US)
Pages (from-to)	345-351
Number of pages	7
Journal	Genetica
Volume	134
Issue number	3
DOIs	https://doi.org/10.1007/s10709-008-9242-1
State	Published - Nov 2008

Keywords

Bos taurus
Homo sapiens
Mammals
Muntiacus muntjak
Muntiacus reevesi
NOR
Synteny

ASJC Scopus subject areas

Insect Science
Genetics
Animal Science and Zoology
Plant Science

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10.1007/s10709-008-9242-1

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title = "Comparative gene mapping in cattle, Indian muntjac, and Chinese muntjac by fluorescence in situ hybridization",

abstract = "The Indian muntjac (Muntiacus muntjak vaginalis) has a karyotype of 2n = 6 in the female and 2n = 7 in the male. The karyotypic evolution of Indian muntjac via extensive tandem fusions and several centric fusions are well documented by molecular cytogenetic studies mainly utilizing chromosome paints. To achieve higher resolution mapping, a set of 42 different genomic clones coding for 37 genes and the nucleolar organizer region were used to examine homologies between the cattle (2n = 60), human (2n = 46), Indian muntjac (2n = 6/7) and Chinese muntjac (2n = 46) karyotypes. These genomic clones were mapped by fluorescence in situ hybridization (FISH). Localization of genes on all three pairs of M. m. vaginalis chromosomes and on the acrocentric chromosomes of M. reevesi allowed not only the analysis of the evolution of syntenic regions within the muntjac genus but also allowed a broader comparison of synteny with more distantly related species, such as cattle and human, to shed more light onto the evolving genome organization.",

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author = "Murmann, {Andrea E.} and Antoaneta Mincheva and Scheuermann, {Markus O.} and Mathieu Gautier and Fentang Yang and Johannes Buitkamp and Strissel, {Pamela L.} and Reiner Strick and Rowley, {Janet D.} and Peter Lichter",

year = "2008",

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doi = "10.1007/s10709-008-9242-1",

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T1 - Comparative gene mapping in cattle, Indian muntjac, and Chinese muntjac by fluorescence in situ hybridization

AU - Murmann, Andrea E.

AU - Mincheva, Antoaneta

AU - Scheuermann, Markus O.

AU - Gautier, Mathieu

AU - Yang, Fentang

AU - Buitkamp, Johannes

AU - Strissel, Pamela L.

AU - Strick, Reiner

AU - Rowley, Janet D.

AU - Lichter, Peter

PY - 2008/11

Y1 - 2008/11

N2 - The Indian muntjac (Muntiacus muntjak vaginalis) has a karyotype of 2n = 6 in the female and 2n = 7 in the male. The karyotypic evolution of Indian muntjac via extensive tandem fusions and several centric fusions are well documented by molecular cytogenetic studies mainly utilizing chromosome paints. To achieve higher resolution mapping, a set of 42 different genomic clones coding for 37 genes and the nucleolar organizer region were used to examine homologies between the cattle (2n = 60), human (2n = 46), Indian muntjac (2n = 6/7) and Chinese muntjac (2n = 46) karyotypes. These genomic clones were mapped by fluorescence in situ hybridization (FISH). Localization of genes on all three pairs of M. m. vaginalis chromosomes and on the acrocentric chromosomes of M. reevesi allowed not only the analysis of the evolution of syntenic regions within the muntjac genus but also allowed a broader comparison of synteny with more distantly related species, such as cattle and human, to shed more light onto the evolving genome organization.

AB - The Indian muntjac (Muntiacus muntjak vaginalis) has a karyotype of 2n = 6 in the female and 2n = 7 in the male. The karyotypic evolution of Indian muntjac via extensive tandem fusions and several centric fusions are well documented by molecular cytogenetic studies mainly utilizing chromosome paints. To achieve higher resolution mapping, a set of 42 different genomic clones coding for 37 genes and the nucleolar organizer region were used to examine homologies between the cattle (2n = 60), human (2n = 46), Indian muntjac (2n = 6/7) and Chinese muntjac (2n = 46) karyotypes. These genomic clones were mapped by fluorescence in situ hybridization (FISH). Localization of genes on all three pairs of M. m. vaginalis chromosomes and on the acrocentric chromosomes of M. reevesi allowed not only the analysis of the evolution of syntenic regions within the muntjac genus but also allowed a broader comparison of synteny with more distantly related species, such as cattle and human, to shed more light onto the evolving genome organization.

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Comparative gene mapping in cattle, Indian muntjac, and Chinese muntjac by fluorescence in situ hybridization

Abstract

Keywords

ASJC Scopus subject areas

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