Comparative localization of receptors for plasmin and for urokinase on MCF7 cells

S. Zhang, M. Laurent, R. Lopez-Alemany, A. Mazar, J. Henkin, E. Ronne, P. Burtin*

*Corresponding author for this work

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

The receptor for plasmin and the receptor for urokinase-type plasminogen activator were characterized on MCF 7 cells either independently or simultaneously, using fluorescence microscopy and con focal microscopy. The plasmin receptor was visualized, as previously described by Correc et al. (Int. J. Cancer 50, 767, 1992) using biotinylated plasminogen and fluoresceinated streptavidin. The urokinase receptor was revealed by both polyclonal and monoclonal antibodies reacting specifically with this receptor and by binding of urokinase aminoterminal fragment. On unfixed cells, these methods gave the same heterogeneous patterns of surface staining, consisting of contours and grains, localized mainly at the upper nonadherent face of the tumor cells by con focal microscopy. Only a part of the cells was stained. When both receptors were characterized together, their presence was found on the same cells and they gave almost superimposable patterns in many cases, as shown by confocal microscopy. In contrast, when MCF 7 cells were fixed or permeabilized before staining, quite different patterns were observed: almost all the cells were labeled. The staining was mainly cytoplasmic and localized preferentially in the center and close to the upper face of the cells. Similar results were found with antiserum against urokinase receptor and monoclonal antivinculin antibody. It is likely that the receptor for urokinase and the receptor for plasmin have similar localizations on MCF 7 cells, thus resulting in a functional cooperation.

Original languageEnglish (US)
Pages (from-to)290-299
Number of pages10
JournalExperimental Cell Research
Volume207
Issue number2
DOIs
StatePublished - Aug 1993

ASJC Scopus subject areas

  • Cell Biology

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    Zhang, S., Laurent, M., Lopez-Alemany, R., Mazar, A., Henkin, J., Ronne, E., & Burtin, P. (1993). Comparative localization of receptors for plasmin and for urokinase on MCF7 cells. Experimental Cell Research, 207(2), 290-299. https://doi.org/10.1006/excr.1993.1196