Comparison of Ca2+ and CaMKII responses in IVF and ICSI in the mouse

Styliani Markoulaki, Manabu Kurokawa, Sara Matson, Tom Ducibella*, Rafael Fissore

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Novel methods of egg activation in human assisted reproductive technologies and animal somatic cell nuclear transfer are likely to alter the signalling process that occurs during normal fertilization. Intracytoplasmic sperm injection (ICSI) bypasses the normal processes of the acrosome reaction, sperm-egg fusion, and processing of the sperm plasma membrane, as well as alters some parameters of intracellular calcium ([Ca2+]i) dynamics (reported previously by Kurokawa and Fissore (2003)). Herein, we extend these studies to determine if ICSI alters the activity of the Ca2+-dependent protein, Ca2+/calmodulin-dependent kinase II (CaMKII), which is responsible for the completion of meiosis in vertebrate eggs. After ICSI or in vitro fertilization (IVF), individual mouse eggs were monitored for their relative changes in both ([Ca2+] i) and CaMKII activity during the first ([Ca2+] i) rise and a subsequent rise associated with second polar body extrusion. The duration of the first ([Ca2+]i) rise was greater in ICSI than in IVF, but the amplitude of the rise was transiently higher for IVF than ICSI. However, a similar mean CaMKII activity was observed in both procedures. During polar body extrusion, the amplitude and duration of the Ca2+ rises were increased by a small amount in ICSI compared with IVF, whereas the CaMKII activities were similar. Thus, compared with IVF, ICSI is not associated with decreased or delayed CaMKII activity in response to these Ca2+ signals in the mouse.

Original languageEnglish (US)
Pages (from-to)265-272
Number of pages8
JournalMolecular human reproduction
Issue number4
StatePublished - Apr 2007


  • Calcium
  • CaMKII
  • Fertilization
  • ICSI
  • IVF

ASJC Scopus subject areas

  • Reproductive Medicine
  • Embryology
  • Molecular Biology
  • Genetics
  • Obstetrics and Gynecology
  • Developmental Biology
  • Cell Biology


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