Comparison of cobe spectra and haemonetics MCS-3P cell separators for peripheral blood stem cell harvesting

J. Mehta*, R. Powles, S. Cabral, V. Shepherd, S. Singhal, R. Morilla, J. Treleaven

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

A prospective study was undertaken to compare the total nucleated cell (TNC), mononuclear cell (MNC), CD34+ cell, and CFU-GM yields of two different cell separators. A Haemonetics MCS-3P and a Cobe Spectra machine were used to leukapherese 10 patients with malignant diseases on 4 consecutive days after mobilization with G-CEF. All patients were harvested twice on each machine for a fixed period of time. The blood volume processed (10.47 vs 3.79 l, P < 0.001), MNC yield (2.66 vs 0.90 x 108/kg; P < 0.001), MNC yield rate (1.66 vs 0.55 x 106/kg/min; P < 0.001), MNC purity (81 vs 42%; P < 0.001), CFU-GM yield (18.1 vs 5.5 x 104/kg; P = 0.001), and CFU-GM yield rate (11.27 vs 3.42 x 102/kg/min; P = 0.001) were significantly higher with the Spectra. The CD34+ cell yields and yield rates were comparable. Although CFU-GM and MNC yields per unit blood volume processed were comparable for both machines, there was a trend for higher CD34+ yields per unit volume processed with MCS-3P. We conclude that Spectra is faster than MCS-3P with more blood processed per unit time resulting in higher cell yields, but yields per unit volume processed are comparable for both machines. The choice of machine for a given patient depends upon convenience, venous access and the time available.

Original languageEnglish (US)
Pages (from-to)707-709
Number of pages3
JournalBone Marrow Transplantation
Volume16
Issue number5
StatePublished - Jan 1 1995

Keywords

  • Cell separators
  • Granulocyte colony-stimulating factor
  • Leukapheresis
  • Peripheral blood stem cell transplantation

ASJC Scopus subject areas

  • Hematology
  • Transplantation

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