Abstract
Purpose: To test the hypothesis that transcatheter arterial embolization (TAE) induces expression of hypoxia-inducible factor-1α (HIF-1α) within the same rabbit VX2 liver tumor. Materials and Methods: Seven VX2 tumors were grown in the livers of five New Zealand white rabbits. Ultrasonography-guided biopsy was performed before and 10 minutes after TAE in all tumors. Pre- and post-TAE tumor biopsy specimens along with post-TAE whole liver tumor sections were stained with HIF-1α antibody and analyzed for percentage of HIF-1α-positive nuclei by using a spectral unmixing system mounted on a high-powered microscope. Statistical data comparisons were performed with the Wilcoxon signed-rank test (α = 0.05). Results: TAE of liver tumors resulted in a statistically significant increase in the mean percentage of HIF-1α expression. The mean percentage of HIF-1α-positive stained nuclei increased from 23% ± 3.5 in pre-TAE biopsy specimens to 41% ± 8.7 in post-TAE biopsy specimens (P < .02). The increase was even more significant when the mean percentage of HIF-1α-positive stained nuclei from the same pre-TAE biopsy specimens was compared with sections from post-TAE whole tumor specimens (60% ± 8.9, P < .02). Conclusions: The results of this study revealed that hypoxia caused by TAE of VX2 liver tumors activates HIF-1α, a transcription factor that in turn regulates other pro-angiogenic factors.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1483-1489 |
| Number of pages | 7 |
| Journal | Journal of Vascular and Interventional Radiology |
| Volume | 19 |
| Issue number | 10 |
| DOIs | |
| State | Published - Oct 1 2008 |
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ASJC Scopus subject areas
- Radiology Nuclear Medicine and imaging
- Cardiology and Cardiovascular Medicine
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Comparison of Hypoxia-inducible Factor-1α Expression before and after Transcatheter Arterial Embolization in Rabbit VX2 Liver Tumors. / Virmani, Sumeet; Rhee, Thomas K.; Ryu, Robert K.; Sato, Kent T.; Lewandowski, Robert J.; Mulcahy, Mary F.; Kulik, Laura M.; Szolc-Kowalska, Barbara; Woloschak, Gayle E.; Yang, Guang Yu; Salem, Riad; Larson, Andrew C.; Omary, Reed A.
In: Journal of Vascular and Interventional Radiology, Vol. 19, No. 10, 01.10.2008, p. 1483-1489.Research output: Contribution to journal › Article
TY - JOUR
T1 - Comparison of Hypoxia-inducible Factor-1α Expression before and after Transcatheter Arterial Embolization in Rabbit VX2 Liver Tumors
AU - Virmani, Sumeet
AU - Rhee, Thomas K.
AU - Ryu, Robert K.
AU - Sato, Kent T.
AU - Lewandowski, Robert J.
AU - Mulcahy, Mary F.
AU - Kulik, Laura M.
AU - Szolc-Kowalska, Barbara
AU - Woloschak, Gayle E.
AU - Yang, Guang Yu
AU - Salem, Riad
AU - Larson, Andrew C.
AU - Omary, Reed A.
PY - 2008/10/1
Y1 - 2008/10/1
N2 - Purpose: To test the hypothesis that transcatheter arterial embolization (TAE) induces expression of hypoxia-inducible factor-1α (HIF-1α) within the same rabbit VX2 liver tumor. Materials and Methods: Seven VX2 tumors were grown in the livers of five New Zealand white rabbits. Ultrasonography-guided biopsy was performed before and 10 minutes after TAE in all tumors. Pre- and post-TAE tumor biopsy specimens along with post-TAE whole liver tumor sections were stained with HIF-1α antibody and analyzed for percentage of HIF-1α-positive nuclei by using a spectral unmixing system mounted on a high-powered microscope. Statistical data comparisons were performed with the Wilcoxon signed-rank test (α = 0.05). Results: TAE of liver tumors resulted in a statistically significant increase in the mean percentage of HIF-1α expression. The mean percentage of HIF-1α-positive stained nuclei increased from 23% ± 3.5 in pre-TAE biopsy specimens to 41% ± 8.7 in post-TAE biopsy specimens (P < .02). The increase was even more significant when the mean percentage of HIF-1α-positive stained nuclei from the same pre-TAE biopsy specimens was compared with sections from post-TAE whole tumor specimens (60% ± 8.9, P < .02). Conclusions: The results of this study revealed that hypoxia caused by TAE of VX2 liver tumors activates HIF-1α, a transcription factor that in turn regulates other pro-angiogenic factors.
AB - Purpose: To test the hypothesis that transcatheter arterial embolization (TAE) induces expression of hypoxia-inducible factor-1α (HIF-1α) within the same rabbit VX2 liver tumor. Materials and Methods: Seven VX2 tumors were grown in the livers of five New Zealand white rabbits. Ultrasonography-guided biopsy was performed before and 10 minutes after TAE in all tumors. Pre- and post-TAE tumor biopsy specimens along with post-TAE whole liver tumor sections were stained with HIF-1α antibody and analyzed for percentage of HIF-1α-positive nuclei by using a spectral unmixing system mounted on a high-powered microscope. Statistical data comparisons were performed with the Wilcoxon signed-rank test (α = 0.05). Results: TAE of liver tumors resulted in a statistically significant increase in the mean percentage of HIF-1α expression. The mean percentage of HIF-1α-positive stained nuclei increased from 23% ± 3.5 in pre-TAE biopsy specimens to 41% ± 8.7 in post-TAE biopsy specimens (P < .02). The increase was even more significant when the mean percentage of HIF-1α-positive stained nuclei from the same pre-TAE biopsy specimens was compared with sections from post-TAE whole tumor specimens (60% ± 8.9, P < .02). Conclusions: The results of this study revealed that hypoxia caused by TAE of VX2 liver tumors activates HIF-1α, a transcription factor that in turn regulates other pro-angiogenic factors.
UR - http://www.scopus.com/inward/record.url?scp=52149116938&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=52149116938&partnerID=8YFLogxK
U2 - 10.1016/j.jvir.2008.06.017
DO - 10.1016/j.jvir.2008.06.017
M3 - Article
C2 - 18922400
AN - SCOPUS:52149116938
VL - 19
SP - 1483
EP - 1489
JO - Journal of Vascular and Interventional Radiology
JF - Journal of Vascular and Interventional Radiology
SN - 1051-0443
IS - 10
ER -