Comparison of methods for evaluating mineral loss: Hardness versus synchrotron microcomputed tomography

A. C.B. Delbem; K. T. Sassaki; A. E.M. Vieira; E. Rodrigues; M. Bergamaschi; S. R. Stock; M. L. Cannon; X. Xiao; F. De Carlo; A. C.B. Delbem

doi:10.1159/000231573

Comparison of methods for evaluating mineral loss: Hardness versus synchrotron microcomputed tomography

A. C.B. Delbem, K. T. Sassaki, A. E.M. Vieira, E. Rodrigues, M. Bergamaschi, S. R. Stock, M. L. Cannon, X. Xiao, F. De Carlo, A. C.B. Delbem

Cell & Developmental Biology

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Abstract

This study analyzed degrees of demineralization in bovine enamel using synchrotron microcomputed tomography (SMCT) and hardness measurements (Knoop hardness number, KHN). For 5 days, 40 bovine enamel blocks were individually subjected to a pH cycling model and treatment with fluoride dentifrices (placebo, 275, 550 and 1,100 μg F/g) diluted in deionized water twice a day. Surface hardness number and cross-sectional profiles of hardness and mineral concentration (by SMCT) were determined. Integrated hardness (KHN × μm) for sound and demineralized specimens was calculated and subtracted to give the integrated loss of hardness (ΔKHN) for the lesions. Increasing fluoride concentration in the dentifrices led to higher values for surface hardness after pH cycling and mineral concentration (g_HAp cm^-3), and lower values for ΔKHN (p < 0.05). From the present results, it may be concluded that hardness measurements revealed demineralization in all groups, which was lower in groups treated with dentifrice with a higher F concentration. SMCT and hardness measurements gave similar results in areas with higher demineralization, but diverged in areas with lower demineralization.

Original language	English (US)
Pages (from-to)	359-365
Number of pages	7
Journal	Caries Research
Volume	43
Issue number	5
DOIs	https://doi.org/10.1159/000231573
State	Published - 2009

Keywords

Bovine enamel
Hardness
In vitro
Synchrotron microcomputed tomography

ASJC Scopus subject areas

Dentistry(all)

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10.1159/000231573

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title = "Comparison of methods for evaluating mineral loss: Hardness versus synchrotron microcomputed tomography",

abstract = "This study analyzed degrees of demineralization in bovine enamel using synchrotron microcomputed tomography (SMCT) and hardness measurements (Knoop hardness number, KHN). For 5 days, 40 bovine enamel blocks were individually subjected to a pH cycling model and treatment with fluoride dentifrices (placebo, 275, 550 and 1,100 μg F/g) diluted in deionized water twice a day. Surface hardness number and cross-sectional profiles of hardness and mineral concentration (by SMCT) were determined. Integrated hardness (KHN × μm) for sound and demineralized specimens was calculated and subtracted to give the integrated loss of hardness (ΔKHN) for the lesions. Increasing fluoride concentration in the dentifrices led to higher values for surface hardness after pH cycling and mineral concentration (gHAp cm-3), and lower values for ΔKHN (p < 0.05). From the present results, it may be concluded that hardness measurements revealed demineralization in all groups, which was lower in groups treated with dentifrice with a higher F concentration. SMCT and hardness measurements gave similar results in areas with higher demineralization, but diverged in areas with lower demineralization.",

keywords = "Bovine enamel, Hardness, In vitro, Synchrotron microcomputed tomography",

author = "Delbem, {A. C.B.} and Sassaki, {K. T.} and Vieira, {A. E.M.} and E. Rodrigues and M. Bergamaschi and Stock, {S. R.} and Cannon, {M. L.} and X. Xiao and {De Carlo}, F. and Delbem, {A. C.B.}",

year = "2009",

doi = "10.1159/000231573",

language = "English (US)",

volume = "43",

pages = "359--365",

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TY - JOUR

T1 - Comparison of methods for evaluating mineral loss

T2 - Hardness versus synchrotron microcomputed tomography

AU - Delbem, A. C.B.

AU - Sassaki, K. T.

AU - Vieira, A. E.M.

AU - Rodrigues, E.

AU - Bergamaschi, M.

AU - Stock, S. R.

AU - Cannon, M. L.

AU - Xiao, X.

AU - De Carlo, F.

AU - Delbem, A. C.B.

PY - 2009

Y1 - 2009

N2 - This study analyzed degrees of demineralization in bovine enamel using synchrotron microcomputed tomography (SMCT) and hardness measurements (Knoop hardness number, KHN). For 5 days, 40 bovine enamel blocks were individually subjected to a pH cycling model and treatment with fluoride dentifrices (placebo, 275, 550 and 1,100 μg F/g) diluted in deionized water twice a day. Surface hardness number and cross-sectional profiles of hardness and mineral concentration (by SMCT) were determined. Integrated hardness (KHN × μm) for sound and demineralized specimens was calculated and subtracted to give the integrated loss of hardness (ΔKHN) for the lesions. Increasing fluoride concentration in the dentifrices led to higher values for surface hardness after pH cycling and mineral concentration (gHAp cm-3), and lower values for ΔKHN (p < 0.05). From the present results, it may be concluded that hardness measurements revealed demineralization in all groups, which was lower in groups treated with dentifrice with a higher F concentration. SMCT and hardness measurements gave similar results in areas with higher demineralization, but diverged in areas with lower demineralization.

AB - This study analyzed degrees of demineralization in bovine enamel using synchrotron microcomputed tomography (SMCT) and hardness measurements (Knoop hardness number, KHN). For 5 days, 40 bovine enamel blocks were individually subjected to a pH cycling model and treatment with fluoride dentifrices (placebo, 275, 550 and 1,100 μg F/g) diluted in deionized water twice a day. Surface hardness number and cross-sectional profiles of hardness and mineral concentration (by SMCT) were determined. Integrated hardness (KHN × μm) for sound and demineralized specimens was calculated and subtracted to give the integrated loss of hardness (ΔKHN) for the lesions. Increasing fluoride concentration in the dentifrices led to higher values for surface hardness after pH cycling and mineral concentration (gHAp cm-3), and lower values for ΔKHN (p < 0.05). From the present results, it may be concluded that hardness measurements revealed demineralization in all groups, which was lower in groups treated with dentifrice with a higher F concentration. SMCT and hardness measurements gave similar results in areas with higher demineralization, but diverged in areas with lower demineralization.

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KW - In vitro

KW - Synchrotron microcomputed tomography

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Comparison of methods for evaluating mineral loss: Hardness versus synchrotron microcomputed tomography

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