Comparison of morphology of human macular and peripheral Bruch's membrane in older eyes

Mark Johnson*, Arun Dabholkar, Jiahn Dar Huang, J. Brett Presley, Melissa F. Chimento, Christine A. Curcio

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


Deposits in macular human Bruch's membrane (BrM) increase with age and have been postulated to be associated with age-related maculopathy. We used two ultrastructural methods to compare these deposits by electron microscopy in macular and peripheral BrM of eight eyes from donors 63-86 years of age. Quick-freeze/deep-etch (QFDE) was used to prepare replicas that showed the ultrastructure of deposits, and osmium-tannic acid-paraphenylenediamine (OTAP) was used to preserve small extracellular lipid particles. We found that an accumulation of lipoprotein-like particles (LLPs) occurred in the peripheral BrM just as it does in the macular region, but with perhaps a somewhat slower time course. The "lipid wall," reported in macular BrM, was also found occasionally in the peripheral regions. The same processes that lead to age-related accumulation of LLPs in macular BrM appear to also occur in the peripheral regions.

Original languageEnglish (US)
Pages (from-to)791-799
Number of pages9
JournalCurrent eye research
Issue number9
StatePublished - Sep 2007


  • AMD
  • Basal lamina
  • Collagen
  • Elastin
  • Lipoprotein
  • Morphology

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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