Comparison of PCR assay to culture for surveillance detection of vancomycin-resistant enterococci

Suzanne M. Paule; William E. Trick; Fred C. Tenover; Mary Lankford; Susan Cunningham; Valentina Stosor; Ralph L. Cordell; Lance R. Peterson

doi:10.1128/JCM.41.10.4805-4807.2003

Comparison of PCR assay to culture for surveillance detection of vancomycin-resistant enterococci

Suzanne M. Paule^*, William E. Trick, Fred C. Tenover, Mary Lankford, Susan Cunningham, Valentina Stosor, Ralph L. Cordell, Lance R. Peterson

^*Corresponding author for this work

Medicine, Infectious Diseases Division

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

Direct multiplex PCR assay using vanA and vanB primers, which provides rapid results, was more sensitive than culture on selective media for samples collected by rectal swab (20 of 46 versus 8 of 46; P < 0.001) or perianal swab (17 of 58 versus 12 of 58; P = 0.059) for the detection of gastrointestinal colonization by vancomycin-resistant enterococci.

Original language	English (US)
Pages (from-to)	4805-4807
Number of pages	3
Journal	Journal of clinical microbiology
Volume	41
Issue number	10
DOIs	https://doi.org/10.1128/JCM.41.10.4805-4807.2003
State	Published - Oct 1 2003

ASJC Scopus subject areas

Microbiology (medical)

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10.1128/JCM.41.10.4805-4807.2003

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abstract = "Direct multiplex PCR assay using vanA and vanB primers, which provides rapid results, was more sensitive than culture on selective media for samples collected by rectal swab (20 of 46 versus 8 of 46; P < 0.001) or perianal swab (17 of 58 versus 12 of 58; P = 0.059) for the detection of gastrointestinal colonization by vancomycin-resistant enterococci.",

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AU - Paule, Suzanne M.

AU - Trick, William E.

AU - Tenover, Fred C.

AU - Lankford, Mary

AU - Cunningham, Susan

AU - Stosor, Valentina

AU - Cordell, Ralph L.

AU - Peterson, Lance R.

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AB - Direct multiplex PCR assay using vanA and vanB primers, which provides rapid results, was more sensitive than culture on selective media for samples collected by rectal swab (20 of 46 versus 8 of 46; P < 0.001) or perianal swab (17 of 58 versus 12 of 58; P = 0.059) for the detection of gastrointestinal colonization by vancomycin-resistant enterococci.

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Comparison of PCR assay to culture for surveillance detection of vancomycin-resistant enterococci

Abstract

ASJC Scopus subject areas

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