Comparison of the inhibitory effects of glucocorticoids on the expression of eotaxin in airway epithelial cell line BEAS-2B

Koushi Ieki, Satoshi Matsukura, Fumio Kokubu, Masatsugu Kurokawa, Mio Kawaguchi, Hideki Kuga, Shin Watanabe, Shintaro Suzuki, Miho Odaka, Hiroko Takeuchi, Robert P. Schleimer, Mitsuru Adachi

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: Inhaled corticosteroids play a pivotal role in the treatment of asthma. To observe the mechanisms of glucocorticoids, we focused our study on the comparison of several glucocorticoids' effects on eotaxin expression in the airway epithelial cells. Methods: Airway epithelial cell line BEAS-2B was cultured in vitro. Cells were preincubated with or without glucocorticoids (becromethasone dipropionate; BDP, budesonide; BUD, fluticasone propionate; FP) and stimulated with TNFα and/or IL-4. Protein levels of eotaxin in the supernatants of the cultured cells were determined by ELISA. Results and Conclusions: TNFα and IL-4 increased the levels of eotaxin in BEAS-2B cells. Combination of these cytokines synergistically upregulated the eotaxin expression as reported previously. Each glucocorticoid significantly inhibited the expression of eotaxin protein induced with TNFα and IL-4 and the compared efficacy was in order of FP>BUD>BDP. FP seemed most potent and the inhibitory effect was also observed with relatively low concentration such as 10- 10 M. Taken together, the comparison of the potency of each glucocorticoid using airway epithelial cells may reflect the efficacy of these drugs in asthmatics.

Original languageEnglish (US)
Pages (from-to)423-429
Number of pages7
JournalJapanese Journal of Allergology
Volume53
Issue number4
StatePublished - Jun 3 2004

Keywords

  • Airway epithelial cell
  • Bronchial asthma
  • Eotaxin
  • Glucocorticoid

ASJC Scopus subject areas

  • Immunology and Allergy

Fingerprint Dive into the research topics of 'Comparison of the inhibitory effects of glucocorticoids on the expression of eotaxin in airway epithelial cell line BEAS-2B'. Together they form a unique fingerprint.

Cite this