Comparison of the rodac imprint method to selective enrichment broth for recovery of vancomycin-resistant enterococci and drug-resistant enterobacteriaceae from environmental surfaces

D. M. Hacek*, W. E. Trick, S. M. Collins, G. A. Noskin, L. R. Peterson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

We compared the Rodac imprint technique to selective enrichment broth for detecting vancomycin-resistant enterococci (VRE) and multidrug-resistant Enterobacteriaceae (MDRE) on surfaces. Rodac plates contained tryptic soy agar with 5% sheep blood, vancomycin (6 μg/ml), ceftazidime (2 μg/ml), amphotericin B (2 μg/ml), and clindamycin (1 μg/ml). Two types of broth were used: brain heart infusion (BHI) and BHI plus vancomycin (6 μg/ml) and ceftazidime (2 μg/ml) (BHIVC). Of the 46 surfaces cultured for VRE, 12 (26%) were positive. Of the 12 VRE-positive surfaces, 11 (92%) grew from Rodac, 8 (67%) grew from BHIVC, and 7 (58%) grew from BHI. A larger study is needed for MDRE, as only 4 of 43 surfaces were MDRE positive. The Rodac imprint technique successfully recovered VRE from environmental surfaces.

Original languageEnglish (US)
Pages (from-to)4646-4648
Number of pages3
JournalJournal of clinical microbiology
Volume38
Issue number12
DOIs
StatePublished - 2000

ASJC Scopus subject areas

  • Microbiology (medical)

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