Comparison of three nonradioisotopic polymerase chain reaction-based methods for detection of human immunodeficiency virus type 1

A. J. Whetsell, J. B. Drew, G. Milman, R. Hoff, E. A. Dragon, K. Adler, J. Hui, P. Otto, P. Gupta, H. Farzadegan, S. M. Wolinsky*

*Corresponding author for this work

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58 Scopus citations

Abstract

Three nonradioisotopic polymerase chain reaction (PCR)-based detection techniques were evaluated for sensitivity and specificity in detecting human immunodeficiency virus type 1 (HIV-1) proviral DNA in peripheral blood mononuclear cells. The Roche prototype HIV-1 PCR assay, the Du Pont enzyme- linked oligonucleotide sandwich assay (ELOSA), and the Gen-Probe hybridization protection assay (HPA) were compared with a standard radioisotopic oligonucleotide solution hybridization (OSH) technique. A panel of 111 well-characterized clinical samples that included peripheral blood mononuclear cells from 48 healthy, low-risk, HIV-1 antibody-negative subjects, 24 antibody-positive subjects with stable CD4 counts of less than 200/mm3, and 39 antibody-positive subjects with stable CD4 counts of greater than 800/mm3 were studied. Each method demonstrated good specificity, ranging between 96 and 100%; those of the OSH and ELOSA (Du Pont) were 100%, those of the HPA (Gen-Probe) were 100% with one probe and 96% with the other probe, and that of the HIV-1 PCR assay (Roche) was 96%. Sensitivities ranged from 96 to 100% for the low-CD4-count group, with the OSH, the HIV-1 PCR assay (Roche), and the HPA (Gen-Probe) all attaining a sensitivity of 100%. For the high-CD4-count group, sensitivities ranged from 69 to 97%, with the OSH attaining a sensitivity of 97% and the HPA attaining sensitivities of 97% with one probe and 95% with the other probe. These data indicate that the nonradioisotopic techniques are sensitive and specific for the detection of HIV-1 proviral DNA in clinical samples.

Original languageEnglish (US)
Pages (from-to)845-853
Number of pages9
JournalJournal of clinical microbiology
Volume30
Issue number4
DOIs
StatePublished - 1992

ASJC Scopus subject areas

  • Microbiology (medical)

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    Whetsell, A. J., Drew, J. B., Milman, G., Hoff, R., Dragon, E. A., Adler, K., Hui, J., Otto, P., Gupta, P., Farzadegan, H., & Wolinsky, S. M. (1992). Comparison of three nonradioisotopic polymerase chain reaction-based methods for detection of human immunodeficiency virus type 1. Journal of clinical microbiology, 30(4), 845-853. https://doi.org/10.1128/jcm.30.4.845-853.1992