Comparison of virulence of Legionella longbeachae strains in guinea pigs and U937 macrophage-like cells

R. M. Doyle*, N. P. Cianciotto, S. Banvi, P. A. Manning, M. W. Heuzenroeder

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

A guinea pig model of experimental legionellosis was established for assessment of virulence of isolates of Legionella longbeachae. The results showed that there were distinct virulence groupings of L. longbeachae serogroup 1 strains based on the severity of disease produced in this model. Statistical analysis of the animal model data suggests that Australian isolates of L. longbeachae may be inherently more virulent than non-Australian strains. Infection studies performed with U937 cells were consistent with the animal model studies and showed that isolates of this species were capable of multiplying within these phagocytic cells. Electron microscopy studies of infected lung tissue were also undertaken to determine the intracellular nature of L. longbeachae serogroup 1 infection. The data showed that phagosomes containing virulent L. longbeachae serogroup 1 appeared bloated, contained cellular debris and had an apparent rim of ribosomes while those containing avirulent L. longbeachae serogroup 1 were compact, clear and smooth.

Original languageEnglish (US)
Pages (from-to)5335-5344
Number of pages10
JournalInfection and immunity
Volume69
Issue number9
DOIs
StatePublished - 2001

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

Fingerprint Dive into the research topics of 'Comparison of virulence of Legionella longbeachae strains in guinea pigs and U937 macrophage-like cells'. Together they form a unique fingerprint.

Cite this