TY - JOUR
T1 - Complementary Embryonic and Adult Cell Populations Enhance Myocardial Repair in Rat Myocardial Injury Model
AU - Li Calzi, Sergio
AU - Cook, Todd
AU - Della Rocca, Domenico G.
AU - Zhang, Juan
AU - Shenoy, Vinayak
AU - Yan, Yuanqing
AU - Espejo, Andrew
AU - Rathinasabapathy, Anandharajan
AU - Jacobsen, Max H.
AU - Salazar, Tatiana
AU - Sandusky, George E.
AU - Shaw, Lynn C.
AU - March, Keith
AU - Raizada, Mohan K.
AU - Pepine, Carl J.
AU - Katovich, Michael J.
AU - Grant, Maria B.
N1 - Funding Information:
Li Calzi Sergio scalzi@uabmc.edu 1 Cook Todd tgcook@iu.edu 2 Della Rocca Domenico G. domenicodellarocca@hotmail.it 3 Zhang Juan zhangjuancwr@outlook.com 4 Shenoy Vinayak vshenoy@chsu.edu 5 Yan Yuanqing yanyuanqing@gmail.com 6 Espejo Andrew andrew.espejo3@gmail.com 4 https://orcid.org/0000-0002-3474-8063 Rathinasabapathy Anandharajan anandharajan.rathinasabapathy@vanderbilt.edu 7 Jacobsen Max H. maxhjaco@iu.edu 8 Salazar Tatiana tso164@gmail.com 1 Sandusky George E. gsandusk@iupui.edu 8 Shaw Lynn C. lshaw1957@gmail.com 1 March Keith keith.march@medicine.ufl.edu 2 Raizada Mohan K. mraizada@phys.med.ufl.edu 5 Pepine Carl J. carl.pepine@medicine.ufl.edu 3 Katovich Michael J. katovich@cop.ufl.edu 4 https://orcid.org/0000-0002-6470-0255 Grant Maria B. mariagrant@uabmc.edu 1 Deutsch Marcus-André 1 Department of Ophthalmology and Visual Sciences University of Alabama at Birmingham Birmingham AL 35294-0001 USA uab.edu 2 Department of Medicine IUPUI Indianapolis IN 46202 USA iupui.edu 3 Department of Medicine University of Florida Gainesville FL 32611 USA ufl.edu 4 Department of Pharmacodynamics University of Florida Gainesville FL 32611 USA ufl.edu 5 Department of Physiology and Functional Genomics University of Florida Gainesville FL 32611 USA ufl.edu 6 Department of Biostatistics University of Texas MD Anderson Cancer Center Houston TX 77030 USA mdanderson.org 7 Allergy, Pulmonary, and Critical Care Medicine Vanderbilt University Medical Center Nashville TN USA vanderbilt.edu 8 Pathology and Laboratory Med. IUPUI Indianapolis IN 46202 USA iupui.edu 2019 3 11 2019 2019 30 11 2018 09 04 2019 01 10 2019 3 11 2019 2019 Copyright © 2019 Sergio Li Calzi et al. This is an open access article distributed under the Creative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. We compared the functional outcome of Isl-1 + cardiac progenitors, CD90 + bone marrow-derived progenitor cells, and the combination of the two in a rat myocardial infarction (MI) model. Isl-1 + cells were isolated from embryonic day 12.5 (E12.5) rat hearts and expanded in vitro. Thy-1 + /CD90 + cells were isolated from the bone marrow of adult Sprague-Dawley rats by immunomagnetic cell sorting. Six-week-old female Sprague-Dawley rats underwent permanent left anterior descending (LAD) coronary artery ligation and received intramyocardial injection of either saline, Isl-1 + cells, CD90 + cells, or a combination of Isl-1 + and CD90 + cells, at the time of infarction. Cells were delivered transepicardially to the peri-infarct zone. Left ventricular function was assessed by transthoracic echocardiography at 1- and 4-week post-MI and by Millar catheterization (-dP/dt and +dP/dt) at 4-week post-MI. Fluorescence in situ hybridization (Isl-1 + cells) and monochrystalline iron oxide nanoparticles labeling (MION; CD90 + cells) were performed to assess biodistribution of transplanted cells. Only the combination of cells demonstrated a significant improvement of cardiac function as assessed by anterior wall contractility, dP/dt (max), and dP/dt (min), compared to Isl-1 + or CD90 + cell monotherapies. In the combination cell group, viable cells were detected at week 4 when anterior wall motion was completely restored. In conclusion, the combination of Isl-1 + cardiac progenitors and adult bone marrow-derived CD90 + cells shows prolonged and robust myocardial tissue repair and provides support for the use of complementary cell populations to enhance myocardial repair. National Institutes of Health DK-09-0730 EY12601 EY07739 HL056921 University of Florida NHLBI 5 UM1 HL08736 American Heart Association 0865213E 0625533B
Publisher Copyright:
© 2019 Sergio Li Calzi et al.
PY - 2019
Y1 - 2019
N2 - We compared the functional outcome of Isl-1+ cardiac progenitors, CD90+ bone marrow-derived progenitor cells, and the combination of the two in a rat myocardial infarction (MI) model. Isl-1+ cells were isolated from embryonic day 12.5 (E12.5) rat hearts and expanded in vitro. Thy-1+/CD90+ cells were isolated from the bone marrow of adult Sprague-Dawley rats by immunomagnetic cell sorting. Six-week-old female Sprague-Dawley rats underwent permanent left anterior descending (LAD) coronary artery ligation and received intramyocardial injection of either saline, Isl-1+ cells, CD90+ cells, or a combination of Isl-1+ and CD90+ cells, at the time of infarction. Cells were delivered transepicardially to the peri-infarct zone. Left ventricular function was assessed by transthoracic echocardiography at 1- and 4-week post-MI and by Millar catheterization (-dP/dt and +dP/dt) at 4-week post-MI. Fluorescence in situ hybridization (Isl-1+cells) and monochrystalline iron oxide nanoparticles labeling (MION; CD90+ cells) were performed to assess biodistribution of transplanted cells. Only the combination of cells demonstrated a significant improvement of cardiac function as assessed by anterior wall contractility, dP/dt (max), and dP/dt (min), compared to Isl-1+ or CD90+ cell monotherapies. In the combination cell group, viable cells were detected at week 4 when anterior wall motion was completely restored. In conclusion, the combination of Isl-1+ cardiac progenitors and adult bone marrow-derived CD90+ cells shows prolonged and robust myocardial tissue repair and provides support for the use of complementary cell populations to enhance myocardial repair.
AB - We compared the functional outcome of Isl-1+ cardiac progenitors, CD90+ bone marrow-derived progenitor cells, and the combination of the two in a rat myocardial infarction (MI) model. Isl-1+ cells were isolated from embryonic day 12.5 (E12.5) rat hearts and expanded in vitro. Thy-1+/CD90+ cells were isolated from the bone marrow of adult Sprague-Dawley rats by immunomagnetic cell sorting. Six-week-old female Sprague-Dawley rats underwent permanent left anterior descending (LAD) coronary artery ligation and received intramyocardial injection of either saline, Isl-1+ cells, CD90+ cells, or a combination of Isl-1+ and CD90+ cells, at the time of infarction. Cells were delivered transepicardially to the peri-infarct zone. Left ventricular function was assessed by transthoracic echocardiography at 1- and 4-week post-MI and by Millar catheterization (-dP/dt and +dP/dt) at 4-week post-MI. Fluorescence in situ hybridization (Isl-1+cells) and monochrystalline iron oxide nanoparticles labeling (MION; CD90+ cells) were performed to assess biodistribution of transplanted cells. Only the combination of cells demonstrated a significant improvement of cardiac function as assessed by anterior wall contractility, dP/dt (max), and dP/dt (min), compared to Isl-1+ or CD90+ cell monotherapies. In the combination cell group, viable cells were detected at week 4 when anterior wall motion was completely restored. In conclusion, the combination of Isl-1+ cardiac progenitors and adult bone marrow-derived CD90+ cells shows prolonged and robust myocardial tissue repair and provides support for the use of complementary cell populations to enhance myocardial repair.
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U2 - 10.1155/2019/3945850
DO - 10.1155/2019/3945850
M3 - Article
C2 - 31781239
AN - SCOPUS:85075528359
SN - 1687-9678
VL - 2019
JO - Stem Cells International
JF - Stem Cells International
M1 - 3945850
ER -