Complete large-molecule high-resolution mass spectra from 50-femtomole microvolume injection

Neil L. Kelleher; Michael W. Senko; Daniel P. Little; Peter B. O'Connor; Fred W. McLafferty

doi:10.1016/1044-0305(95)00088-U

Complete large-molecule high-resolution mass spectra from 50-femtomole microvolume injection

Neil L. Kelleher, Michael W. Senko, Daniel P. Little, Peter B. O'Connor, Fred W. McLafferty^*

^*Corresponding author for this work

Molecular Biosciences

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

For electrospray ionization in Fourier-transform mass spectrometry, direct injection of 5 × 10⁻¹⁴ mol (0.5 μL of 100 nM from a microvolume sample valve) of ubiquitin (8565 Da) into the flowing solvent stream yields a spectrum with 85:1 signal-to-noise ratio, 2-ppm mass accuracy, and isotopic resolution. Gated trapping for 100 μs from a 0.15-μL/min injection of 20-μM ubiquitin consumes 5 × 10⁻¹⁸ mol, which produces a spectrum with 23:1 signal-to-noise ratio and 〉 3 × 10⁵ resolving power.

Original language	English (US)
Pages (from-to)	220-221
Number of pages	2
Journal	Journal of the American Society for Mass Spectrometry
Volume	6
Issue number	3
DOIs	https://doi.org/10.1016/1044-0305(95)00088-U
State	Published - 1995

ASJC Scopus subject areas

Structural Biology
Spectroscopy

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@article{f48441a79bd2462aa42c42daefd4a849,

title = "Complete large-molecule high-resolution mass spectra from 50-femtomole microvolume injection",

abstract = "For electrospray ionization in Fourier-transform mass spectrometry, direct injection of 5 × 10−14 mol (0.5 μL of 100 nM from a microvolume sample valve) of ubiquitin (8565 Da) into the flowing solvent stream yields a spectrum with 85:1 signal-to-noise ratio, 2-ppm mass accuracy, and isotopic resolution. Gated trapping for 100 μs from a 0.15-μL/min injection of 20-μM ubiquitin consumes 5 × 10−18 mol, which produces a spectrum with 23:1 signal-to-noise ratio and 〉 3 × 105 resolving power.",

author = "Kelleher, {Neil L.} and Senko, {Michael W.} and Little, {Daniel P.} and O'Connor, {Peter B.} and McLafferty, {Fred W.}",

note = "Funding Information: The authors thank Troy D. Wood, Keith L. Goodman, J. Paul Speir, and Russell A. Chorush for helpful discussions and suggestions. We also acknowledge the National Institutes of Health for generous financial support under grant GM-16609.",

year = "1995",

doi = "10.1016/1044-0305(95)00088-U",

language = "English (US)",

volume = "6",

pages = "220--221",

journal = "Journal of the American Society for Mass Spectrometry",

issn = "1044-0305",

publisher = "Springer New York",

number = "3",

}

TY - JOUR

T1 - Complete large-molecule high-resolution mass spectra from 50-femtomole microvolume injection

AU - Kelleher, Neil L.

AU - Senko, Michael W.

AU - Little, Daniel P.

AU - O'Connor, Peter B.

AU - McLafferty, Fred W.

N1 - Funding Information: The authors thank Troy D. Wood, Keith L. Goodman, J. Paul Speir, and Russell A. Chorush for helpful discussions and suggestions. We also acknowledge the National Institutes of Health for generous financial support under grant GM-16609.

PY - 1995

Y1 - 1995

N2 - For electrospray ionization in Fourier-transform mass spectrometry, direct injection of 5 × 10−14 mol (0.5 μL of 100 nM from a microvolume sample valve) of ubiquitin (8565 Da) into the flowing solvent stream yields a spectrum with 85:1 signal-to-noise ratio, 2-ppm mass accuracy, and isotopic resolution. Gated trapping for 100 μs from a 0.15-μL/min injection of 20-μM ubiquitin consumes 5 × 10−18 mol, which produces a spectrum with 23:1 signal-to-noise ratio and 〉 3 × 105 resolving power.

AB - For electrospray ionization in Fourier-transform mass spectrometry, direct injection of 5 × 10−14 mol (0.5 μL of 100 nM from a microvolume sample valve) of ubiquitin (8565 Da) into the flowing solvent stream yields a spectrum with 85:1 signal-to-noise ratio, 2-ppm mass accuracy, and isotopic resolution. Gated trapping for 100 μs from a 0.15-μL/min injection of 20-μM ubiquitin consumes 5 × 10−18 mol, which produces a spectrum with 23:1 signal-to-noise ratio and 〉 3 × 105 resolving power.

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