Complete large-molecule high-resolution mass spectra from 50-femtomole microvolume injection

Neil L. Kelleher, Michael W. Senko, Daniel P. Little, Peter B. O'Connor, Fred W. McLafferty*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

For electrospray ionization in Fourier-transform mass spectrometry, direct injection of 5 × 10−14 mol (0.5 μL of 100 nM from a microvolume sample valve) of ubiquitin (8565 Da) into the flowing solvent stream yields a spectrum with 85:1 signal-to-noise ratio, 2-ppm mass accuracy, and isotopic resolution. Gated trapping for 100 μs from a 0.15-μL/min injection of 20-μM ubiquitin consumes 5 × 10−18 mol, which produces a spectrum with 23:1 signal-to-noise ratio and 〉 3 × 105 resolving power.

Original languageEnglish (US)
Pages (from-to)220-221
Number of pages2
JournalJournal of the American Society for Mass Spectrometry
Volume6
Issue number3
DOIs
StatePublished - 1995

Funding

The authors thank Troy D. Wood, Keith L. Goodman, J. Paul Speir, and Russell A. Chorush for helpful discussions and suggestions. We also acknowledge the National Institutes of Health for generous financial support under grant GM-16609.

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy

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