Complete large-molecule high-resolution mass spectra from 50-femtomole microvolume injection

Neil L. Kelleher, Michael W. Senko, Daniel P. Little, Peter B. O'Connor, Fred W. McLafferty*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

For electrospray ionization in Fourier-transform mass spectrometry, direct injection of 5 × 10−14 mol (0.5 μL of 100 nM from a microvolume sample valve) of ubiquitin (8565 Da) into the flowing solvent stream yields a spectrum with 85:1 signal-to-noise ratio, 2-ppm mass accuracy, and isotopic resolution. Gated trapping for 100 μs from a 0.15-μL/min injection of 20-μM ubiquitin consumes 5 × 10−18 mol, which produces a spectrum with 23:1 signal-to-noise ratio and 〉 3 × 105 resolving power.

Original languageEnglish (US)
Pages (from-to)220-221
Number of pages2
JournalJournal of the American Society for Mass Spectrometry
Volume6
Issue number3
DOIs
StatePublished - 1995

ASJC Scopus subject areas

  • Structural Biology
  • Spectroscopy

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