Abstract
For electrospray ionization in Fourier-transform mass spectrometry, direct injection of 5 × 10−14 mol (0.5 μL of 100 nM from a microvolume sample valve) of ubiquitin (8565 Da) into the flowing solvent stream yields a spectrum with 85:1 signal-to-noise ratio, 2-ppm mass accuracy, and isotopic resolution. Gated trapping for 100 μs from a 0.15-μL/min injection of 20-μM ubiquitin consumes 5 × 10−18 mol, which produces a spectrum with 23:1 signal-to-noise ratio and 〉 3 × 105 resolving power.
Original language | English (US) |
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Pages (from-to) | 220-221 |
Number of pages | 2 |
Journal | Journal of the American Society for Mass Spectrometry |
Volume | 6 |
Issue number | 3 |
DOIs | |
State | Published - 1995 |
Funding
The authors thank Troy D. Wood, Keith L. Goodman, J. Paul Speir, and Russell A. Chorush for helpful discussions and suggestions. We also acknowledge the National Institutes of Health for generous financial support under grant GM-16609.
ASJC Scopus subject areas
- Structural Biology
- Spectroscopy