Abstract
For electrospray ionization in Fourier-transform mass spectrometry, direct injection of 5 × 10−14 mol (0.5 μL of 100 nM from a microvolume sample valve) of ubiquitin (8565 Da) into the flowing solvent stream yields a spectrum with 85:1 signal-to-noise ratio, 2-ppm mass accuracy, and isotopic resolution. Gated trapping for 100 μs from a 0.15-μL/min injection of 20-μM ubiquitin consumes 5 × 10−18 mol, which produces a spectrum with 23:1 signal-to-noise ratio and 〉 3 × 105 resolving power.
Original language | English (US) |
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Pages (from-to) | 220-221 |
Number of pages | 2 |
Journal | Journal of the American Society for Mass Spectrometry |
Volume | 6 |
Issue number | 3 |
DOIs | |
State | Published - 1995 |
ASJC Scopus subject areas
- Structural Biology
- Spectroscopy