Comprehensive genetic testing identifies targetable genomic alterations in most patients with non-small cell lung cancer, specifically adenocarcinoma, single institute investigation

Janani Vigneswaran, Yi Hung Carol Tan, Septimiu D. Murgu, Brian M. Won, Kathryn Alexa Patton, Victoria M. Villaflor, Philip C. Hoffman, Thomas Hensing, D. Kyle Hogarth, Renuka Malik, Heber MacMahon, Jeffrey Mueller, Cassie A. Simon, Wickii T. Vigneswaran, Christopher H. Wigfield, Mark K. Ferguson, Aliya N. Husain, Everett E. Vokes, Ravi Salgia*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

This study reviews extensive genetic analysis in advanced non-small cell lung cancer (NSCLC) patients in order to: Describe how targetable mutation genes interrelate with the genes identified as variants of unknown significance; assess the percentage of patients with a potentially targetable genetic alterations; evaluate the percentage of patients who had concurrent alterations, previously considered to be mutually exclusive; and characterize the molecular subset of KRAS. Thoracic Oncology Research Program Databases at the University of Chicago provided patient demographics, pathology, and results of genetic testing. 364 patients including 289 adenocarcinoma underwent genotype testing by various platforms such as FoundationOne, Caris Molecular Intelligence, and Response Genetics Inc. For the entire adenocarcinoma cohort, 25% of patients were African Americans; 90% of KRAS mutations were detected in smokers, including current and former smokers; 46% of EGFR and 61% of ALK alterations were detected in never smokers. 99.4% of patients, whose samples were analyzed by next-generation sequencing (NGS), had genetic alterations identified with an average of 10.8 alterations/tumor throughout different tumor subtypes. However, mutations were not mutually exclusive. NGS in this study identified potentially targetable genetic alterations in the majority of patients tested, detected concurrent alterations and provided information on variants of unknown significance at this time but potentially targetable in the future.

Original languageEnglish (US)
Pages (from-to)18876-18886
Number of pages11
JournalOncotarget
Volume7
Issue number14
DOIs
StatePublished - 2016

Keywords

  • Genetic testing
  • Genomic alteration
  • Next-generation sequencing
  • Non-small cell lung cancer

ASJC Scopus subject areas

  • Oncology

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