Computational analyses of mammalian lactate dehydrogenases: Human, mouse, opossum and platypus LDHs

Roger S. Holmes*, Erwin Goldberg

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

Computational methods were used to predict the amino acid sequences and gene locations for mammalian lactate dehydrogenase (LDH) genes and proteins using genome sequence databanks. Human LDHA, LDHC and LDH6A genes were located in tandem on chromosome 11, while LDH6B and LDH6C genes were on chromosomes 15 and 12, respectively. Opossum LDHC and LDH6B genes were located in tandem with the opossum LDHA gene on chromosome 5 and contained 7 (LDHA and LDHC) or 8 (LDH6B) exons. An amino acid sequence prediction for the opossum LDH6B subunit gave an extended N-terminal sequence, similar to the human and mouse LDH6B sequences, which may support the export of this enzyme into mitochondria. The platypus genome contained at least 3 LDH genes encoding LDHA, LDHB and LDH6B subunits. Phylogenetic studies and sequence analyses indicated that LDHA, LDHB and LDH6B genes are present in all mammalian genomes examined, including a monotreme species (platypus), whereas the LDHC gene may have arisen more recently in marsupial mammals.

Original languageEnglish (US)
Pages (from-to)379-385
Number of pages7
JournalComputational Biology and Chemistry
Volume33
Issue number5
DOIs
StatePublished - Oct 2009

Keywords

  • Amino acid sequence
  • Genomics
  • Lactate dehydrogenase
  • Mammals
  • Opossum
  • Platypus

ASJC Scopus subject areas

  • Computational Mathematics
  • Structural Biology
  • Biochemistry
  • Organic Chemistry

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