Computational analysis of alternative polyadenylation in embryonic stem cells and induced pluripotent cells

Zhe Ji, Mainul Hoque, Bin Tian*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The 3' untranslated region (3'UTR) of mRNA plays important roles in posttranscriptional control of gene expression. Over half of the human genes have multiple polyadenylation sites in 3'UTRs, leading to 3'UTR isoforms containing different cis elements. Alternative polyadenylation (APA) has been found to be dynamically regulated in different tissue types and under various cellular conditions. Embryonic stem (ES) cells have the ability to self-renew and differentiate into any cell type in the adult body. Posttranscriptional gene regulation through cis elements in 3'UTRs is increasingly found to be important for these functions. In addition, various methods have recently been developed to induce differentiated cells to ES-like cells, called induced pluripotent stem (iPS) cells. Here we show a computational method to examine regulation of 3'UTR by APA using DNA microarray data. We applied this method to ES cells and iPS cells derived from different cell types.

Original languageEnglish (US)
Title of host publicationComputational Biology of Embryonic Stem Cells
PublisherBentham Science Publishers Ltd.
Pages133-146
Number of pages14
ISBN (Print)9781608054343
DOIs
StatePublished - Dec 1 2012

Keywords

  • 3' end formation
  • 3'UTR
  • Alternative polyadenylation
  • Development
  • Differentiation
  • Embryonic stem cells
  • Induced pluripotent cells
  • MicroRNA
  • Post-transcriptional gene regulation
  • Proliferation
  • mRNA isoform
  • mRNA processing

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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