Abstract
The 3' untranslated region (3'UTR) of mRNA plays important roles in posttranscriptional control of gene expression. Over half of the human genes have multiple polyadenylation sites in 3'UTRs, leading to 3'UTR isoforms containing different cis elements. Alternative polyadenylation (APA) has been found to be dynamically regulated in different tissue types and under various cellular conditions. Embryonic stem (ES) cells have the ability to self-renew and differentiate into any cell type in the adult body. Posttranscriptional gene regulation through cis elements in 3'UTRs is increasingly found to be important for these functions. In addition, various methods have recently been developed to induce differentiated cells to ES-like cells, called induced pluripotent stem (iPS) cells. Here we show a computational method to examine regulation of 3'UTR by APA using DNA microarray data. We applied this method to ES cells and iPS cells derived from different cell types.
Original language | English (US) |
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Title of host publication | Computational Biology of Embryonic Stem Cells |
Publisher | Bentham Science Publishers Ltd. |
Pages | 133-146 |
Number of pages | 14 |
ISBN (Print) | 9781608054343 |
DOIs | |
State | Published - Dec 1 2012 |
Keywords
- 3' end formation
- 3'UTR
- Alternative polyadenylation
- Development
- Differentiation
- Embryonic stem cells
- Induced pluripotent cells
- MicroRNA
- Post-transcriptional gene regulation
- Proliferation
- mRNA isoform
- mRNA processing
ASJC Scopus subject areas
- General Biochemistry, Genetics and Molecular Biology