Computationally reconstructing cotranscriptional RNA folding from experimental data reveals rearrangement of non-native folding intermediates

Angela M. Yu, Paul M. Gasper, Luyi Cheng, Lien B. Lai, Simi Kaur, Venkat Gopalan, Alan A. Chen*, Julius B. Lucks

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Experimentally guided multi-scale modeling of the cotranscriptional folding pathway of the E. coli signal recognition particle (SRP) RNA reveals that it undergoes an internal toehold-mediated strand-displacement process that requires local structural fluctuations. An intermediate of this pathway is a substrate for RNase P, suggesting concurrent transcription and processing.

Original languageEnglish (US)
Pages (from-to)870-883.e10
JournalMolecular cell
Volume81
Issue number4
DOIs
StatePublished - Feb 18 2021

Keywords

  • cotranscriptional RNA folding
  • molecular dynamics
  • RNA
  • RNA fluctuations
  • RNA folding dynamics
  • RNA structure
  • SHAPE-seq
  • signal recognition particle
  • toehold strand displacement
  • transcription

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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