Abstract
The construction of a completely synthetic gene offers the most flexible way to synthesize proteins (smaller than ~50,000 molecular weight) with any desired features. The design process of synthetic gene experiments involves a large amount of information, such as the deoxyribonucleotide sequences (primary structure of genes) and amino-acid sequences (the primary structure of proteins), the codon table (the relation between the DNA triplets and amino acids), restriction endonuclease map (mapping of restriction endonuclease sites in a gene sequence), and so on. The ambiguity derived from the reverse translation (conversion of amino acids to proper DNA triplets) further increases the complexity of the design process. A prototype system which is able to manage the information and guide biologists toward a workable experiment design has been developed. The domain knowledge is incorporated into the system by using a knowledge-based expert-system shell, LISP, and C procedural routines on a SUN workstation. An intelligent, graphical user interface has been developed to ease biologists' interaction with the workstation. The system can reduce the experiment design process to hours.
Original language | English (US) |
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Title of host publication | IEEE/Engineering in Medicine and Biology Society Annual Conference |
Publisher | Publ by IEEE |
Pages | 1440-1441 |
Number of pages | 2 |
Volume | 10 pt 3 |
State | Published - Dec 1 1988 |
Event | Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society - New Orleans, LA, USA Duration: Nov 4 1988 → Nov 7 1988 |
Other
Other | Proceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society |
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City | New Orleans, LA, USA |
Period | 11/4/88 → 11/7/88 |
ASJC Scopus subject areas
- Engineering(all)