Computer-assisted planning of the completely synthetic gene experimental design

K. Jiang*, J. Zheng, S. B. Higgins, D. M. Watterson

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

The construction of a completely synthetic gene offers the most flexible way to synthesize proteins (smaller than ~50,000 molecular weight) with any desired features. The design process of synthetic gene experiments involves a large amount of information, such as the deoxyribonucleotide sequences (primary structure of genes) and amino-acid sequences (the primary structure of proteins), the codon table (the relation between the DNA triplets and amino acids), restriction endonuclease map (mapping of restriction endonuclease sites in a gene sequence), and so on. The ambiguity derived from the reverse translation (conversion of amino acids to proper DNA triplets) further increases the complexity of the design process. A prototype system which is able to manage the information and guide biologists toward a workable experiment design has been developed. The domain knowledge is incorporated into the system by using a knowledge-based expert-system shell, LISP, and C procedural routines on a SUN workstation. An intelligent, graphical user interface has been developed to ease biologists' interaction with the workstation. The system can reduce the experiment design process to hours.

Original languageEnglish (US)
Title of host publicationIEEE/Engineering in Medicine and Biology Society Annual Conference
PublisherPubl by IEEE
Pages1440-1441
Number of pages2
Volume10 pt 3
StatePublished - Dec 1 1988
EventProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society - New Orleans, LA, USA
Duration: Nov 4 1988Nov 7 1988

Other

OtherProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society
CityNew Orleans, LA, USA
Period11/4/8811/7/88

ASJC Scopus subject areas

  • Engineering(all)

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