Concanavalin a binding and cytodifferentiation in pancreatic acinar carcinoma of rat

John R. Warren*, Michael J. Trump, Janardan K. Reddy

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

The binding of concanavalin A to the plasmalemma of acinar carcinoma cells was characterized by electron microscopy utilizing horseradish peroxidase. Heavy labeling due to specific concanavalin A binding was detected on the plasmalemma of undifferentiated carcinoma cells lacking zymogen maturation, neoplastic cells of intermediate differentiation with only occasional zymogen granules, and highly differentiated acinar carcinoma cells containing numerous cytoplasmic zymogen granules. The plasmalemma of acinar carcinoma cells was also compared to the normal pancreatic acinar cell plasmalemma by measurement of specific 125I-labeled concanavalin A binding. Although only about one-third of pancreatic acinar carcinoma cells demonstrate mature zymogen differentiation, the acinar carcinoma had a full complement of normal plasmalemma receptors for 125I-labeled concanavalin A. It is concluded that, unlike normal pancreas, the presence of concanavalin A receptors on the plasmalemma of acinar carcinoma cells is not a specific membrane marker for differentiated cells containing zymogen granules.

Original languageEnglish (US)
Pages (from-to)50-56
Number of pages7
JournalBBA - Biomembranes
Volume690
Issue number1
DOIs
StatePublished - Aug 25 1982

Keywords

  • (Rat pancreatic acinar carcinoma)
  • Concanavalin A binding
  • Cytodifferentiation
  • Membrane receptor
  • Plasmalemma
  • Zymogen granule

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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