Concentration-dependent effects of dentin phosphophoryn in the regulation of in vitro hydroxyapatite formation and growth

Adele L. Boskey*, Michael Maresca, Steven Doty, Boris Sabsay, Arthur Veis

*Corresponding author for this work

Research output: Contribution to journalArticle

154 Scopus citations

Abstract

The effect of dentin phosphophoryn on hydroxyapatite formation and growth was studied in an in vitro gelatin gel diffusion system. Phosphophoryn, in low concentrations (0.010-1 μg/ml) promoted de novo hydroxyapatite formation; at a higher concentration (100 μg/ml) in the same system, the dentin matrix protein inhibited hydroxyapatite growth. Similar inhibition of hydroxyapatite growth was seen in solution. The intact phosphophoryn was not essential for either inhibition of seeded growth or promotion of mineralization, since the formic acid degraded protein was comparably effective. Transmission electron microscopy of the precipitates formed at 7 days showed no significant differences in crystallite size distribution in the presence and absence of phosphophoryn. However there was a dose-dependent decrease in the number of mineral clusters formed in the presence of increasing amounts of phosphophoryn, suggesting inhibition of secondary nucleation. These data provide support for the postulated 'multifunctional' role of the dentin phosphoprotein in the mineralization process.

Original languageEnglish (US)
Pages (from-to)55-65
Number of pages11
JournalBone and Mineral
Volume11
Issue number1
DOIs
StatePublished - Oct 1990

Keywords

  • Dentin
  • Dentin phosphoprotein
  • Hydroxyapatite
  • Mineralization
  • Phosphophoryn

ASJC Scopus subject areas

  • Surgery
  • Biochemistry
  • Endocrinology

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