Concurrent acetylcholinesterase staining and γ-aminobutyric acid uptake of cortical neurons in culture

M. M. Mesulam, M. Dichter

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Gamma-aminobutyric acid (GABA) uptake and acetylcholinesterase (AChE) content were demonstrated concurrently in cortical neurons grown in tissue culture. Positive reactions either for GABA uptake or for AChE content were encountered in pyramidal and stellate, as well as spindle-shaped neurons. Neither reaction was confined to a specific morphological subtype. Nearly half the neurons were negative for either reaction. Most of the remaining neurons were positive only for GABA or only for AChE. However, a subpopulation of neurons showed not only a high AChE content, but also an avid GABA uptake. Thus, four types of neurons could be identified on the basis of these two reactions. The high AChE content in some of the cortical neurons that also showed GABA uptake indicates that there are at least two distinct types of GABAergic neurons.

Original languageEnglish (US)
Pages (from-to)306-308
Number of pages3
JournalJournal of Histochemistry and Cytochemistry
Volume29
Issue number2
DOIs
StatePublished - 1981

ASJC Scopus subject areas

  • Anatomy
  • Histology

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