Conditional deletion of Gata3 shows its essential function in TH1-TH2 responses

Jinfang Zhu*, Booki Min, Jane Hu-Li, Cynthia J. Watson, Alex Grinberg, Qi Wang, Nigel Killeen, Joseph F. Urban, Liying Guo, William E. Paul

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

448 Scopus citations

Abstract

Expression of the transcription factor GATA-3 is strongly associated with T helper type 2 (TH2) differentiation, but genetic evidence for its involvement in this process has been lacking. Here, we generated a conditional GATA-3-deficient mouse line. In vitro deletion of Gata3 diminished both interleukin 4 (IL-4)-dependent and IL-4-independent TH2 cell differentiation; without GATA-3, TH1 differentiation occurred in the absence of IL-12 and interferon-γ. Gata3 deletion limited the growth of TH2 cells but not TH1 cells. Deletion of Gata3 from established TH2 cells abolished IL-5 and IL-13 but not IL-4 production. In vivo deletion of Gata3 using OX40-Cre eliminated TH2 responses and allowed the development of interferon-γ-producing cells in mice infected with Nippostrongylus brasiliensis. Thus, GATA-3 serves as a principal switch in determining TH1-TH2 responses.

Original languageEnglish (US)
Pages (from-to)1157-1165
Number of pages9
JournalNature Immunology
Volume5
Issue number11
DOIs
StatePublished - Nov 2004
Externally publishedYes

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Conditional deletion of Gata3 shows its essential function in T<sub>H</sub>1-T<sub>H</sub>2 responses'. Together they form a unique fingerprint.

Cite this