Confocal microscopy using an InGaN violet laser diode at 406nm

J. M. Girkin*, A. I. Ferguson, D. L. Wokosin, A. M. Gurney

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

We report on the application of a novel all-solid-state violet laser diode source to confocal microscopy. The source has the potential to replace argon ion lasers in a range of fluorescence based imaging systems. Improvements in system performance and image quality through the use of anamorphic prisms to modify the beam profile have been characterised. These modifications have permitted high quality, optically sectioned images to be obtained from laser diodes operating around 406nm. Living mammalian cells stained with a range of biologically significant fluorophores have been imaged. In addition, it has been shown that at this wavelength it is possible to image dyes that normally require excitation with UV argon laser lines.

Original languageEnglish (US)
Pages (from-to)336-341
Number of pages6
JournalOptics Express
Volume7
Issue number10
DOIs
StatePublished - Nov 2000

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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